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Literature summary extracted from

  • Ji, J.H.; Yang, J.S.; Hur, J.W.
    Purification and characterization of the exo-beta -D-glucosaminidase from Aspergillus flavus IAM2044 (2003), J. Microbiol. Biotechnol., 13, 269-275.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.165 45000
-
 gel filtrration Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Aspergillus flavus
-
-
-
3.2.1.165 Aspergillus flavus IAM2044
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165
-
 Aspergillus flavus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.165 culture filtrate
-
 Aspergillus flavus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.165 1167
-
-
 Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 chitobiose + H2O
-
 Aspergillus flavus D-glucosamine
-
 ?
3.2.1.165 chitobiose + H2O
-
 Aspergillus flavus IAM2044 D-glucosamine
-
 ?
3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus flavus D-glucosamine
-
 ?
3.2.1.165 chitohexaose + H2O chitohexaose is hydrolyzed to chitopentaose and D-glucosamine at the initial stage of the reaction. The final product is D-glucosamine Aspergillus flavus IAM2044 D-glucosamine
-
 ?
3.2.1.165 chitopentaose + H2O
-
 Aspergillus flavus D-glucosamine
-
 ?
3.2.1.165 chitopentaose + H2O
-
 Aspergillus flavus IAM2044 D-glucosamine
-
 ?
3.2.1.165 chitosan + H2O
-
 Aspergillus flavus D-glucosamine + ?
-
 ?
3.2.1.165 chitosan + H2O
-
 Aspergillus flavus IAM2044 D-glucosamine + ?
-
 ?
3.2.1.165 chitotetraose + H2O
-
 Aspergillus flavus D-glucosamine
-
 ?
3.2.1.165 chitotriose + H2O
-
 Aspergillus flavus D-glucosamine
-
 ?
3.2.1.165 glycol chitosan + H2O
-
 Aspergillus flavus ?
-
 ?
3.2.1.165 glycol chitosan + H2O
-
 Aspergillus flavus IAM2044 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.165 monomer 1 * 45000, SDS-PAGE Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 50
-
-
 Aspergillus flavus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.165 30 60 30°C: about 70% of maximal activity, 60°C: about 45% of maximal activity Aspergillus flavus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.165 50
-
 15 min, stable below Aspergillus flavus
3.2.1.165 60
-
 15 min, about 80% loss of activity Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 5
-
-
 Aspergillus flavus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.165 4 6 pH 4.0: about 55% of maximal activity, pH 6.0: about 60% of maximal activity Aspergillus flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.165 3.5 7 37°C, 15 min, more than 50% of initial activity remains Aspergillus flavus