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Literature summary extracted from

  • Eydoux, C.; De Caro, J.; Ferrato, F.; Boullanger, P.; Lafont, D.; Laugier, R.; Carriere, F.; De Caro, A.
    Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells (2007), J. Lipid Res., 48, 1539-1549.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Colipase
-
 Homo sapiens
3.1.1.3 sodium taurodeoxycholate
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expression of PLRP2 in the protease A-deficient yeast Pichia pastoris as secreted enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates the phospholipase A1 activity of the enzyme, overview Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 49946
-
 x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens
3.1.1.3 50000
-
 x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens
3.1.1.3 52343
-
 x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant secreted enzyme from protease A-deficient Pichia pastoris strain culture supernatant by cation and anion exchange chromatography to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 550
-
 purified recombinant enzyme in presence of colipase at pH 8.8 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information the enzyme also shows phospholipase A1 activity, EC 3.1.1.32, galactolipase activity, EC 3.1.1.26, and acylglycerol lipase activity, EC 3.1.1.23, overview Homo sapiens ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Homo sapiens dibutyrin + butyrate
-
 ?
3.1.1.3 trioctanoin + H2O
-
 Homo sapiens dioctanoin + octanoate
-
 ?
3.1.1.3 triolein + H2O
-
 Homo sapiens diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 50000, recombinant enzyme, SDS-PAGE, x * 52343, recombinant glycosylated enzyme, mass spectrometry, x * 49946, recombinant nonglycosylated enzyme, mass spectrometry Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 HPLRP2
-
 Homo sapiens
3.1.1.3 pancreatic lipase-related protein 2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8 9
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 additional information
-
 pH profiles of the recombinant enzyme, overview Homo sapiens