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Literature summary extracted from

  • Liao, Y.; An, K.; Zhou, X.; Chen, W.; Kuai, B.
    AtCLH2, a typical but possibly distinctive chlorophyllase gene in Arabidopsis (2007), J. Integr. Plant Biol., 49, 531-539.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 acetone activates at lower concentration, Chlase2 reaches its maximal activity in 40% acetone, in 40-60% acetone the activity is reduced, overview Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.14 gene chl2, expression anaylsis, recombinant expression in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.14 additional information inhibition of expression of AtCLH2 by RNA interference, transfection using Agrobacterium tumefaciens strain LBA4404, AtCLH2 RNAi plants show decreased contents of chlorophyllide without a substantial change in the total amount of the extractable chlorophyll and consequently presented lower chlorophyllide to chlorophyll ratios in their leaves, phenotype, overview Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 acetone activates at lower concentration, Chlase2 reaches its maximal activity in 40% acetone, in 40-60% acetone the activity is reduced, overview Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.14 35000
-
 x * 35000, recombinant AtCLH2, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 chlorophyll + H2O Arabidopsis thaliana the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview phytol + chlorophyllide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Arabidopsis thaliana Q9M7I7 isozyme AtCLH2; ecotype Columbia, isozyme AtCLH2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.14 recombinant isozyme AtCLH2 from Escherichia coli strain BL21 (DE3) Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.14 cauline leaf
-
 Arabidopsis thaliana
-
3.1.1.14 flower
-
 Arabidopsis thaliana
-
3.1.1.14 flower bud
-
 Arabidopsis thaliana
-
3.1.1.14 leaf
-
 Arabidopsis thaliana
-
3.1.1.14 additional information spatial and temporal expression patterns of AtCLH2 in wild type plants, overview Arabidopsis thaliana
-
3.1.1.14 rosette leaf
-
 Arabidopsis thaliana
-
3.1.1.14 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll + H2O
-
 Arabidopsis thaliana phytol + chlorophyllide
-
 ?
3.1.1.14 chlorophyll + H2O the enzyme is involved in the first step of chlorophyll degradation, molecular regulation of in vivo activities, AtCLH2 might play a distinctive role in chlorophyll catabolism in vivo, overview Arabidopsis thaliana phytol + chlorophyllide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.14 ? x * 35000, recombinant AtCLH2, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.1.14 AtCLH2
-
 Arabidopsis thaliana
3.1.1.14 Chlase2
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.14 40
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 7
-
 assay at Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.14 6 9 isozyme AtCLH2 Arabidopsis thaliana