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Literature summary extracted from

  • Gundllapalli, S.B.; Pretorius, I.S.; Cordero Otero, R.R.
    Effect of the cellulose-binding domain on the catalytic activity of a beta-glucosidase from Saccharomycopsis fibuligera (2007), J. Ind. Microbiol. Biotechnol., 34, 413-421.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression of wild-type and mutant hybrid of BGL1-CBD2 domain with cellobiohydrolase CBHII in in Saccharomyces cerevisiae strain CENPK42 under the control of phosphoglycerate-kinase-1 promoter PGK1P and terminator PGK1T and yeast mating pheromone alpha-factor secretion signal MFalpha1S, subcloning in Escherichia coli Saccharomycopsis fibuligera
3.2.1.91 the cellulose-binding domain 2 of CBHII is fused to the beta-glucosidase enzyme from Saccharomycopsis fibuligera and expressed in Saccharomyces cerevisiae strain CENPK42 Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 additional information fusion of BGL1 to the cellulose-binding domain CBD2 of Trichoderma reesei cellobiohydrolase CBHII, recombinant enzymes are constructed with single and double copies of CBD2 fused at the N-terminus of BGL1 to mimic the two-domain organization displayed by cellulolytic enzymes in nature, the mutant shows a 2-4fold increased activity compared to the wild-type enzyme Saccharomycopsis fibuligera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 additional information
-
 additional information kinetics of wild-type and mutant enzymes Saccharomycopsis fibuligera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 220000
-
 recombinant wild-type enzyme Saccharomycopsis fibuligera
3.2.1.21 225000 230000 recombinant mutant enzyme Saccharomycopsis fibuligera

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Saccharomycopsis fibuligera
-
-
-
3.2.1.91 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 DEAE Sepharose column chromatography Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 additional information
-
-
 Saccharomycopsis fibuligera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Saccharomycopsis fibuligera 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 avicel + H2O
-
 Saccharomycopsis fibuligera ?
-
 ?
3.2.1.21 cellobiose + H2O
-
 Saccharomycopsis fibuligera 2 beta-D-glucose
-
 ?
3.2.1.21 cellopentaose + H2O
-
 Saccharomycopsis fibuligera 5 D-glucose
-
 ?
3.2.1.21 cellotriose + H2O
-
 Saccharomycopsis fibuligera 3 D-glucose
-
 ?
3.2.1.21 additional information BGL1 is a non-cellulolytic yeast enzyme, substrate specifiicty with beta-1,4 linkage-specific cellulosic substrates, overview Saccharomycopsis fibuligera ?
-
 ?
3.2.1.91 avicel + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.91 carboxymethylcellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BGL1
-
 Saccharomycopsis fibuligera
3.2.1.91 CBHII
-
 Trichoderma reesei
3.2.1.91 cellobiohydrolase
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 22
-
 assay at room temperature, substrate cellobiose Saccharomycopsis fibuligera
3.2.1.21 37
-
 assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside Saccharomycopsis fibuligera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 additional information
-
 temperature profile Saccharomycopsis fibuligera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
 assay at, substrate cellobiose Saccharomycopsis fibuligera
3.2.1.21 5
-
 assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside Saccharomycopsis fibuligera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 additional information
-
 pH profile Saccharomycopsis fibuligera