EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.80 | expressed in Pichia pastoris strain X33 | Lolium perenne |
3.2.1.153 | expression in Pichia pastoris | Lolium perenne |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.80 | additional information | glucose and fructose (20 mM) do not affect 1-FEHa activity | Lolium perenne | |
3.2.1.80 | sucrose | 90% inhibition at 20 mM | Lolium perenne | |
3.2.1.153 | sucrose | - |
Lolium perenne |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.153 | additional information | Lolium perenne | high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.80 | Lolium perenne | Q64GB3 | variant Bravo | - |
3.2.1.153 | Lolium perenne | Q64GB3 | L. var. Bravo | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.80 | Sephadex G50 gel filtration | Lolium perenne |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.80 | leaf | - |
Lolium perenne | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.80 | 1,1-kestotetraose + H2O | 100% activity | Lolium perenne | ? | - |
? | |
3.2.1.80 | 1-kestotriose + H2O | 58% activity compared to 1,1-kestotetraose | Lolium perenne | ? | - |
? | |
3.2.1.80 | 6-kestotriose + H2O | 6% activity compared to 1,1-kestotetraose | Lolium perenne | ? | - |
? | |
3.2.1.80 | 6G-kestotriose + H2O | 19% activity compared to 1,1-kestotetraose | Lolium perenne | ? | - |
? | |
3.2.1.80 | inulin + 5 H2O | 24% activity compared to 1,1-kestotetraose | Lolium perenne | 5 beta-D-fructofuranose + alpha-D-glucopyranose | - |
? | |
3.2.1.80 | levan + H2O | 0.5% activity compared to 1,1-kestotetraose | Lolium perenne | D-fructose + ? | - |
? | |
3.2.1.80 | additional information | no hydrolase activity towards sucrose and loliose | Lolium perenne | ? | - |
? | |
3.2.1.153 | 1,1-kestotetraose + H2O | - |
Lolium perenne | ? | - |
? | |
3.2.1.153 | 1-kestotriose + H2O | 58% of the activity with 1,1-kestotetraose | Lolium perenne | ? | - |
? | |
3.2.1.153 | inulin + H2O | 24 of the activity with 1,1-kestotetraose | Lolium perenne | ? | - |
? | |
3.2.1.153 | additional information | high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes | Lolium perenne | ? | - |
? | |
3.2.1.153 | additional information | low activity against 6-kestotriose and levan. No hydrolase activity can be detected towards sucrose | Lolium perenne | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.80 | 1-FEHa | - |
Lolium perenne |
3.2.1.80 | EC 3.2.1.153 | formerly | Lolium perenne |
3.2.1.80 | fructan 1-exohydrolase | - |
Lolium perenne |
3.2.1.153 | 1-FEH | - |
Lolium perenne |
3.2.1.153 | Lp1-FEHa | - |
Lolium perenne |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.153 | 40 | - |
- |
Lolium perenne |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.153 | 30 | 50 | 30°C: about 70% of maximal activity, 50°C: about 30% of maximal activity | Lolium perenne |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.80 | 4 | 50 | 20% of the maximal activity is still detected at 4°C, 1-FEHa activity decreases strongly above 40°C and falls to 20% of ist maximal activity at 50°C | Lolium perenne |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.80 | 5.5 | - |
- |
Lolium perenne |
3.2.1.153 | 5.5 | - |
- |
Lolium perenne |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.153 | 4.3 | 6.3 | pH 4.3: about 60% of maximal activity, pH 6.3: about 50% of maximal activity | Lolium perenne |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.153 | 2.8 | - |
sucrose | - |
Lolium perenne |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.80 | Lolium perenne | isoelectric focusing | - |
5.2 |
3.2.1.153 | Lolium perenne | - |
- |
5.2 |