Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization (2007), J. Exp. Bot., 58, 1969-1983.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 expressed in Pichia pastoris strain X33 Lolium perenne
3.2.1.153 expression in Pichia pastoris Lolium perenne

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 additional information glucose and fructose (20 mM) do not affect 1-FEHa activity Lolium perenne
3.2.1.80 sucrose 90% inhibition at 20 mM Lolium perenne
3.2.1.153 sucrose
-
Lolium perenne

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.153 additional information Lolium perenne high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Lolium perenne Q64GB3 variant Bravo
-
3.2.1.153 Lolium perenne Q64GB3 L. var. Bravo
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 Sephadex G50 gel filtration Lolium perenne

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 leaf
-
Lolium perenne
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 1,1-kestotetraose + H2O 100% activity Lolium perenne ?
-
?
3.2.1.80 1-kestotriose + H2O 58% activity compared to 1,1-kestotetraose Lolium perenne ?
-
?
3.2.1.80 6-kestotriose + H2O 6% activity compared to 1,1-kestotetraose Lolium perenne ?
-
?
3.2.1.80 6G-kestotriose + H2O 19% activity compared to 1,1-kestotetraose Lolium perenne ?
-
?
3.2.1.80 inulin + 5 H2O 24% activity compared to 1,1-kestotetraose Lolium perenne 5 beta-D-fructofuranose + alpha-D-glucopyranose
-
?
3.2.1.80 levan + H2O 0.5% activity compared to 1,1-kestotetraose Lolium perenne D-fructose + ?
-
?
3.2.1.80 additional information no hydrolase activity towards sucrose and loliose Lolium perenne ?
-
?
3.2.1.153 1,1-kestotetraose + H2O
-
Lolium perenne ?
-
?
3.2.1.153 1-kestotriose + H2O 58% of the activity with 1,1-kestotetraose Lolium perenne ?
-
?
3.2.1.153 inulin + H2O 24 of the activity with 1,1-kestotetraose Lolium perenne ?
-
?
3.2.1.153 additional information high expression level in conditions of active fructan synthesis, low expression level when fructan contents are low. The enzyme might play a role as a beta-(2,1) trimming enzyme acting during fructan synthesis in concert with fructan synthesis enzymes Lolium perenne ?
-
?
3.2.1.153 additional information low activity against 6-kestotriose and levan. No hydrolase activity can be detected towards sucrose Lolium perenne ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 1-FEHa
-
Lolium perenne
3.2.1.80 EC 3.2.1.153 formerly Lolium perenne
3.2.1.80 fructan 1-exohydrolase
-
Lolium perenne
3.2.1.153 1-FEH
-
Lolium perenne
3.2.1.153 Lp1-FEHa
-
Lolium perenne

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.153 40
-
-
Lolium perenne

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.153 30 50 30°C: about 70% of maximal activity, 50°C: about 30% of maximal activity Lolium perenne

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 4 50 20% of the maximal activity is still detected at 4°C, 1-FEHa activity decreases strongly above 40°C and falls to 20% of ist maximal activity at 50°C Lolium perenne

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5.5
-
-
Lolium perenne
3.2.1.153 5.5
-
-
Lolium perenne

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.153 4.3 6.3 pH 4.3: about 60% of maximal activity, pH 6.3: about 50% of maximal activity Lolium perenne

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.153 2.8
-
sucrose
-
Lolium perenne

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.80 Lolium perenne isoelectric focusing
-
5.2
3.2.1.153 Lolium perenne
-
-
5.2