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Literature summary extracted from

  • Shinomiya, K.; Kobayashi, H.; Inokuchi, N.; Kobayashi, K.; Oshima, H.; Kitanaka, S.; Yanagidaira, K.; Sasaki, H.; Muto, M.; Okano, M.; Ito, Y.
    New small-scale cross-axis coil planet centrifuge. Partition efficiency and application to purification of bullfrog ribonuclease (2007), J. Chromatogr. A, 1151, 91-98.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.6.1.18 analysis studies on application to purify ribonuclease from eggs of Rana catesbeiana with an aqueous–aqueous polymer phase system by using a small-scale cross-axis coil planet centrifuge Lithobates catesbeianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.18 RNA + H2O Lithobates catesbeianus
-
 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Lithobates catesbeianus P14626 bullfrog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 gel filtration of crude egg extracts, a two-phase polymer system composed of 12.5% PEG 1000 and 12.5% dibasic potassium phosphate added to crude extracts prior to further purification by small-scale cross-axis coil planet centrifuge Lithobates catesbeianus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 egg
-
 Lithobates catesbeianus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 RNA + H2O
-
 Lithobates catesbeianus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
4.6.1.18 RNA + H2O studies on application to the purification of ribonuclease of Rana catesbeiana eggs using an aqueous-aqueous polymer phase system and a small-scale cross-axis coil planet centrifuge Lithobates catesbeianus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 pancreatic ribonuclease
-
 Lithobates catesbeianus
4.6.1.18 ribonuclease A
-
 Lithobates catesbeianus