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Literature summary extracted from

  • Elliott, T.W.; Mitic, N.; Gahan, L.R.; Guddat, L.W.; Schenk, G.
    Inhibition studies of purple acid phosphatases: implications for the catalytic mechanism (2006), J. Braz. Chem. Soc., 17, 1558-1565.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 fluoride competitive inhibitor independent of pH Phaseolus vulgaris
3.1.3.2 fluoride competitive inhibitor independent of pH Sus scrofa
3.1.3.2 vanadate
-
 Phaseolus vulgaris
3.1.3.2 vanadate non-competitive inhibitor at low pH and non-competitive inhibitor at higher pH Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 2.6
-
 p-nitrophenyl phosphate at pH 4.9 Phaseolus vulgaris
3.1.3.2 3.7
-
 p-nitrophenyl phosphate at pH 4.9 Sus scrofa
3.1.3.2 5.9
-
 p-nitrophenyl phosphate at pH 6.2 Phaseolus vulgaris
3.1.3.2 7
-
 p-nitrophenyl phosphate at pH 6.2 Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Phaseolus vulgaris
-
 variety red kidney bean
-
3.1.3.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 CM-cellulose resin chromatography and Sephadex G-75 gel filtration Sus scrofa
3.1.3.2 CM-cellulose resin chromatography and Sephadex S-300 gel filtration Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 fruit
-
 Phaseolus vulgaris
-
3.1.3.2 uterine fluid
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Phaseolus vulgaris p-nitrophenol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Sus scrofa p-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 PAP
-
 Phaseolus vulgaris
3.1.3.2 PAP
-
 Sus scrofa
3.1.3.2 purple acid phosphatase
-
 Phaseolus vulgaris
3.1.3.2 purple acid phosphatase
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.9
-
-
 Sus scrofa
3.1.3.2 6.2
-
-
 Phaseolus vulgaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 0.0295
-
 vanadate competitive inhibition at pH 6.2 Phaseolus vulgaris
3.1.3.2 0.04
-
 vanadate at pH 5.5 Sus scrofa
3.1.3.2 1.1
-
 fluoride competitive inhibition at pH 6.2 Phaseolus vulgaris
3.1.3.2 5.99
-
 fluoride competitive inhibition at pH 6.2 Sus scrofa