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Literature summary extracted from

  • Kang, J.H.; Hwang, Y.; Yoo, O.J.
    Expression of penicillin G acylase gene from Bacillus megaterium ATCC 14945 in Escherichia coli and Bacillus subtilis (1991), J. Biotechnol., 17, 99-108.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.11 synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expressed in Escherichia coli and Bacillus subtilis, high expression rate of extracellular enzyme in Bacillus subtilis Priestia megaterium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 extracellular
-
 Priestia megaterium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.11 57000
-
 2 * 57000, SDS-PAGE, native mass by gel filtration Priestia megaterium
3.5.1.11 115000
-
 gel filtration Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.11 penicillin G + H2O Priestia megaterium
-
 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Priestia megaterium
-
 strain ATCC 14945
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.11 from cell culture medium of transgenic Bacillus subtilis Priestia megaterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.11 0.19
-
 ampicillin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 0.23
-
 cephradine as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 0.56
-
 cephalexin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 0.67
-
 cephaloglycin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 1.24
-
 cephaloridine as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 1.36
-
 cefamandole as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 1.58
-
 cephalothin as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium
3.5.1.11 1.86
-
 penicillin G as subsrate, purified enzyme, 37°C, pH 6.8 Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 7-amino-desacetoxycephalosporanic acid + D-alpha-phenylglycine methylester acylating activity Priestia megaterium ?
-
 ?
3.5.1.11 7-aminocephalosporanic acid + D-alpha-phenylglycine methylester acylating activity Priestia megaterium ?
-
 ?
3.5.1.11 cefamandole + H2O
-
 Priestia megaterium (6R,7R)-7-amino-3-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-hydroxy(phenyl)acetic acid
-
 ?
3.5.1.11 cephalexin + H2O
-
 Priestia megaterium (2R)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.1.11 cephaloglycin + H2O
-
 Priestia megaterium ?
-
 ?
3.5.1.11 cephaloridine + H2O
-
 Priestia megaterium (6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
-
 ?
3.5.1.11 cephalothin + H2O
-
 Priestia megaterium (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
 ?
3.5.1.11 cephradine + H2O
-
 Priestia megaterium (2R)-amino(cyclohexa-1,4-dien-1-yl)acetic acid + (6R,7R)-7-amino-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
 ?
3.5.1.11 additional information no activity with cephapirin, cefotaximen cephalosporin, and penicillin N Priestia megaterium ?
-
 ?
3.5.1.11 penicillin G + H2O
-
 Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.11 dimer 2 * 57000, SDS-PAGE, native mass by gel filtration Priestia megaterium

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin G acylase
-
 Priestia megaterium
3.5.1.11 PGA
-
 Priestia megaterium