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Literature summary extracted from

  • Lin Teng Shee, F.; Arul, J.; Brunet, S.; Bazinet, L.
    Effect of bipolar membrane electrobasification on chitosanase activity during chitosan hydrolysis (2008), J. Biotechnol., 134, 305-311.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Streptomyces sp.
-
-
-
3.2.1.132 Streptomyces sp. 174
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O chitosan of approximately 100 kDa and degree of deacetylation 96% Streptomyces sp. ?
-
?
3.2.1.132 chitosan + H2O chitosan of approximately 100 kDa and degree of deacetylation 96% Streptomyces sp. 174 ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5.5
-
-
Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 additional information
-
study on the interaction of pH value with activity. Reaction rate decreases by 50% from pH 5.5 to 6.0 Streptomyces sp.
3.2.1.132 6
-
loss of 50% of activity Streptomyces sp.
3.2.1.132 7
-
above, complete loss of activity due to partial denaturation of enzyme and chitosan substrate insolubilization Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 2
-
loss of 90% of activity after 1 h incubation Streptomyces sp.
3.2.1.132 3 8 stable for at least 7 h Streptomyces sp.
3.2.1.132 9
-
and above, loss of 50% of activity after 1 h incubation Streptomyces sp.