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Literature summary extracted from

  • Goedl, C.; Schwarz, A.; Minani, A.; Nidetzky, B.
    Recombinant sucrose phosphorylase from Leuconostoc mesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilised enzyme for production of alpha-D-glucose 1-phosphate (2007), J. Biotechnol., 129, 77-86.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.7 synthesis LmSPase is immobilised onto Eupergit C and used for the continuous production of alpha-D-glucose 1-phosphate from sucrose Leuconostoc mesenteroides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.7 DNA and amino acid sequence determination and analysis, genetic structure, 60fold overexpression of LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide, with the sequence Arg-Gly-Ser-His6-Gly-Ser, in Escherichia coli DH10B Leuconostoc mesenteroides
2.4.1.7 expression of His-tagged enzyme in Escherichia coli DH10B Leuconostoc mesenteroides

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.7 additional information immobilization of the purified recombinant tagged enzyme for continuous production of alpha-D-glucose 1-phosphate from sucrose and phosphate in a packed bed reactor, method optimization, overview Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.7 additional information
-
additional information kinetics Leuconostoc mesenteroides
2.4.1.7 5.7
-
sucrose pH 7.0, 30°C, recombinant enzyme Leuconostoc mesenteroides
2.4.1.7 9.5
-
phosphate pH 7.0, 30°C, recombinant enzyme Leuconostoc mesenteroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.7 55000
-
x * 55000 Leuconostoc mesenteroides
2.4.1.7 60000
-
x * 60000, SDS-PAGE Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.7 sucrose + phosphate Leuconostoc mesenteroides
-
D-fructose + alpha-D-glucose 1-phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Leuconostoc mesenteroides
-
-
-
2.4.1.7 Leuconostoc mesenteroides Q59495
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7
-
Leuconostoc mesenteroides
2.4.1.7 recombinant LmSPase containing an 11 amino acid-long N-terminal metal affinity fusion peptide from Escherichia coli DH10B 7fold to homogeneity by metal affinity and hydrophobic interaction chromatography Leuconostoc mesenteroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.7 190
-
-
Leuconostoc mesenteroides
2.4.1.7 190
-
purified recombinant tagged enzyme Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 alpha-D-glucose 1-phosphate + D-arabitol transglucosylation Leuconostoc mesenteroides ?
-
?
2.4.1.7 alpha-D-glucose 1-phosphate + D-arabitol soluble recombinant enzyme Leuconostoc mesenteroides phosphate + alpha-D-glucosyl-D-arabitol
-
?
2.4.1.7 alpha-D-glucose 1-phosphate + L-arabitol soluble recombinant enzyme Leuconostoc mesenteroides phosphate + alpha-D-glucosyl-L-arabitol
-
?
2.4.1.7 alpha-L-glucose 1-phosphate + D-arabitol transglucosylation Leuconostoc mesenteroides ?
-
?
2.4.1.7 sucrose + phosphate
-
Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
?
2.4.1.7 sucrose + phosphate
-
Leuconostoc mesenteroides D-fructose + alpha-D-glucose 1-phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.7 ? x * 60000, SDS-PAGE Leuconostoc mesenteroides
2.4.1.7 ? x * 55000 Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 LmSPase
-
Leuconostoc mesenteroides
2.4.1.7 SPase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 30
-
assay at Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.7 22 45
-
Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.7 30
-
maximal stability Leuconostoc mesenteroides
2.4.1.7 30
-
purified recombinant enzyme, stable below Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.7 96
-
alpha-D-glucose 1-phosphate pH 7.0, 30°C, synthesis, recombinant enzyme Leuconostoc mesenteroides
2.4.1.7 165
-
sucrose pH 7.0, 30°C, phosphorolysis, recombinant enzyme Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.5 7 both reaction directions Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.7 5.5 7.5
-
Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.7 7 7.5 maximal stability Leuconostoc mesenteroides
2.4.1.7 7 7.5 purified recombinant enzyme, completely stable Leuconostoc mesenteroides