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Literature summary extracted from

  • Chen, X.; Cao, Y.; Ding, Y.; Lu, W.; Li, D.
    Cloning, functional expression and characterization of Aspergillus sulphureus beta-mannanase in Pichia pastoris (2007), J. Biotechnol., 128, 452-461.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Pichia pastoris Aspergillus sulphureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
 additional information Km value for locust bean gum at pH 2.4, 50°C is 0.93 mg/ml Aspergillus sulphureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular
-
 Aspergillus sulphureus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 39000
-
 x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme Aspergillus sulphureus
3.2.1.78 41389
-
 x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme Aspergillus sulphureus
3.2.1.78 48000
-
 x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme Aspergillus sulphureus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Aspergillus sulphureus Q2LE69
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein treatment with Endo H leads to 30% loss of enzymic activity and a reduction in molecular weight by about 9 kDa Aspergillus sulphureus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 culture fluid
-
 Aspergillus sulphureus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 366
-
 pH 2.4, 50°C Aspergillus sulphureus

Storage Stability

EC Number Storage Stability Organism
3.2.1.78 enzyme is stable at room temperature for at least 2 days Aspergillus sulphureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Aspergillus sulphureus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 41389, calculated, x * 48000, SDS-PAGE of glycosylated enzyme, x * 39000, SDS-PAGE of deglycosylated enzyme Aspergillus sulphureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
 Aspergillus sulphureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 40
-
 stable below Aspergillus sulphureus
3.2.1.78 60
-
 half-life 50 min Aspergillus sulphureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 2.4
-
-
 Aspergillus sulphureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 2.2 8
-
 Aspergillus sulphureus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.78 Aspergillus sulphureus isoelectric focusing
-
 4.9