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Literature summary extracted from
- Some distinguishable properties between acid-stable and neutral types of alpha-amylases from acid-producing koji (2007), J. Biosci. Bioeng., 104, 353-362.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | KSCN | 500 mM, the reaction rate of neutral amylase increases markedly | Aspergillus niger |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | acid-stable amylase | Aspergillus niger |
| 3.2.1.1 | neutral amylase | Aspergillus niger |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | KSCN | 500 mM, the reaction rate of acid-stable amylase decreases | Aspergillus niger | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Aspergillus awamori | - |
- |
- |
| 3.2.1.1 | Aspergillus luchuensis | - |
- |
- |
| 3.2.1.1 | Aspergillus niger | - |
- |
- |
| 3.2.1.1 | Cryptococcus sp. (in: Fungi) | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | acid-stable amylase | Aspergillus niger |
| 3.2.1.1 | neutral amylase | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | 2-chloro-4-nitrophenyl-alpha-maltotrioside + H2O | acid-stable amylase and common neutral amylase can degrade the substrate at pH 5.4 to release 2-chloro-4-nitrophenol. In presence of 500 mM KSCN the reaction rate of common neutral amylase increases markedly whereas the reaction rate of acid-stable amylase decreases | Aspergillus niger | 2-chloro-4-nitrophenol + alpha-maltotrioside | - |
? | |
| 3.2.1.1 | 2-chloro-4-nitrophenyl-alpha-maltotrioside + H2O | acid-stable amylase and common neutral amylase can degrade the substrate at pH 5.4 to release 2-chloro-4-nitrophenol. In presence of 500 mM KSCN the reaction rate of common neutral amylase increases markedly whereas the reaction rate of acid-stable amylase decreases | Aspergillus awamori | 2-chloro-4-nitrophenol + alpha-maltotrioside | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | acid-stable amylase | - |
Aspergillus niger |
| 3.2.1.1 | neutral amylase | - |
Aspergillus niger |
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Release 2023.1 (January 2023)
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