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Literature summary extracted from

  • Rattanakit, N.; Yano, S.; Plikomol, A.; Wakayama, M.; Tachiki, T.
    Purification of Aspergillus sp. S1-13 chitinases and their role in saccharification of chitin in mash of solid-state culture with shellfish waste (2007), J. Biosci. Bioeng., 103, 535-541.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 p-nitrophenyl-GlcNAc 10 mM, 50% inhibition Aspergillus sp.
3.2.1.52 GlcNAc exochitinase Aspergillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 45000
-
 1 * 45000, endochitinase-1, SDS-PAGE Aspergillus sp.
3.2.1.14 51000
-
 1 * 51000, endochitinase-2, SDS-PAGE Aspergillus sp.
3.2.1.52 73000
-
 1 * 73000, exochitinase, SDS-PAGE Aspergillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Aspergillus sp.
-
 S1-13
-
3.2.1.14 Aspergillus sp. S1-13
-
 S1-13
-
3.2.1.52 Aspergillus sp.
-
 S1-13
-
3.2.1.52 Aspergillus sp. S1-13
-
 S1-13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Aspergillus sp.
3.2.1.52
-
 Aspergillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 1.1
-
-
 Aspergillus sp.
3.2.1.14 8
-
-
 Aspergillus sp.
3.2.1.52 2.2
-
-
 Aspergillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 colloidal chitin + H2O
-
 Aspergillus sp. ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Aspergillus sp. S1-13 ?
-
 ?
3.2.1.14 ethylene glycol chitin + H2O low activity Aspergillus sp. ?
-
 ?
3.2.1.14 ethylene glycol chitin + H2O low activity Aspergillus sp. S1-13 ?
-
 ?
3.2.1.14 lactic acid-treated crab shell + H2O 13% of the activity with colloidal chitin Aspergillus sp. ?
-
 ?
3.2.1.14 lactic acid-treated crab shell + H2O 18% of the activity with colloidal chitin Aspergillus sp. ?
-
 ?
3.2.1.14 additional information endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc Aspergillus sp. ?
-
 ?
3.2.1.14 additional information endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose Aspergillus sp. ?
-
 ?
3.2.1.14 additional information endochitinase-1 is inert to N,N',N''-triacetylchitotriose, N,N'-diacetylchitobiose and p-nitrophenyl-GlcNAc Aspergillus sp. S1-13 ?
-
 ?
3.2.1.14 additional information endochitinase-2 is not active towards ethylene glycol chitin, low activity towards N-acetylchitoologosaccharides, no hydrolysis og N,N'-diacetylchitobiose Aspergillus sp. S1-13 ?
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Aspergillus sp. N,N',N''-triacetylchitotriose + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Aspergillus sp. N,N'-diacetylchitobiose + GlcNAc
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Aspergillus sp. 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Aspergillus sp. S1-13 2 N,N'-diacetylchitobiose
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O
-
 Aspergillus sp. N,N'-diacetylchitobiose + GlcNAc
-
 ?
3.2.1.14 powder chitin + H2O
-
 Aspergillus sp. ?
-
 ?
3.2.1.52 additional information exochitinase is not active with colloidal chitin, powder chitin and lactic acid-treated crab shell Aspergillus sp. ?
-
 ?
3.2.1.52 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
 ?
3.2.1.52 p-nitrophenyl-GlcNAc + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
 ?
3.2.1.52 p-nitrophenyl-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
 ?
3.2.1.52 p-nitrophenyl-N,N',N'',N'''-tetraacetylchitotetraose + H2O exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
 ?
3.2.1.52 p-nitrophenyl-N,N'-diacetylchitobiose + H2O best substrate, exochitinase Aspergillus sp. p-nitrophenol + GlcNAc
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 45000, endochitinase-1, SDS-PAGE Aspergillus sp.
3.2.1.14 monomer 1 * 51000, endochitinase-2, SDS-PAGE Aspergillus sp.
3.2.1.52 monomer 1 * 73000, exochitinase, SDS-PAGE Aspergillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 endochitinase-1
-
 Aspergillus sp.
3.2.1.14 endochitinase-2
-
 Aspergillus sp.
3.2.1.52 exochitinase
-
 Aspergillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37
-
 endochitinase-1 Aspergillus sp.
3.2.1.14 45
-
 endochitinase-2 Aspergillus sp.
3.2.1.52 45
-
 exochitinase Aspergillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 1 50 pH 5.5, 10 min, endochitinase-1, stable Aspergillus sp.
3.2.1.14 1 50 pH 5.5, 10 min, endochitinase-2, stable Aspergillus sp.
3.2.1.14 60
-
 pH 5.5, 10 min, endochitinase-1, 50% loss of activity Aspergillus sp.
3.2.1.14 70
-
 pH 5.5, 10 min, endochitinase-2, 40% loss of activity Aspergillus sp.
3.2.1.52 60
-
 pH 5.5, 10 min, exochitinase, no loss of activity Aspergillus sp.
3.2.1.52 70
-
 pH 5.5, 10 min, exochitinase, 50% loss of activity Aspergillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5 5.5 endochitinase-1 Aspergillus sp.
3.2.1.14 5 5.5 endochitinase-2 Aspergillus sp.
3.2.1.52 5 5.5 exochitinase Aspergillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4.5 6.5 stable, endochitinase-1 Aspergillus sp.
3.2.1.14 4.5 6.5 stable, endochitinase-2 Aspergillus sp.
3.2.1.52 4.5 6.5 stable, exochitinase Aspergillus sp.