Any feedback?
Literature summary extracted from
- Purification and some properties of beta-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404 (2007), J. Biosci. Bioeng., 103, 491-493.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.26 | additional information | not inhibited by metal ions | Aureobasidium pullulans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.26 | 230 | - |
sucrose | in 50 mM sodium acetate buffer (pH 5.0), at 30°C | Aureobasidium pullulans |  |
| 3.2.1.26 | 700 | - |
1-kestose | in 50 mM sodium acetate buffer (pH 5.0), at 30°C | Aureobasidium pullulans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 430000 | - |
TSK Super SW3000 column HPLC | Aureobasidium pullulans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.26 | Aureobasidium pullulans | - |
strain DSM 2404 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.26 | DEAE-cellulose DE52 column chromatography, Toyopearl butyl-650M column chromatography, and Sepharose CL-4B column chromatography | Aureobasidium pullulans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 42.4 | - |
crude extract, at 30°C | Aureobasidium pullulans |
| 3.2.1.26 | 2180 | - |
after 51.4fold purification, at 30°C | Aureobasidium pullulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.26 | 1-kestose + H2O | - |
Aureobasidium pullulans | ? | - |
? | |
| 3.2.1.26 | sucrose + H2O | - |
Aureobasidium pullulans | D-glucose + D-fructose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.26 | beta-fructofuranosidase I | - |
Aureobasidium pullulans |
| 3.2.1.26 | FFase I | - |
Aureobasidium pullulans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 50 | - |
- |
Aureobasidium pullulans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 45 | - |
stable at 45°C for 1 h | Aureobasidium pullulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 5 | - |
- |
Aureobasidium pullulans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.26 | 6 | 10 | stable at pH 6-10 at 30°C for 1 h | Aureobasidium pullulans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
