EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | chloroform | at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h | Pseudomonas aeruginosa | |
3.1.1.3 | dimethylformamide | at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h | Pseudomonas aeruginosa | |
3.1.1.3 | DTT | 20% activation at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | EDTA | 30% activation at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | iodoacetic acid | 10% activation at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | sodium deoxycholate | 300% activation at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Triton X-100 | 300% activation at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Tween 80 | 200% activation at 5 mM | Pseudomonas aeruginosa |
EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.3 | biotechnology | the alkaline lipase is stable and active in organic solvents and a water-restricted environment and has a great potential as a biotechnological tool e.g. in organosynthetic reactions in water-restricted medium or in control and prevention of metalworking fluid putrification in the metal industry | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | 50% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Cu2+ | 92% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | diisopropyl fluorophosphate | 30% inhibition at 5 mM, 60% inhibition at 25 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Hg2+ | 94% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Mg2+ | 32% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Mn2+ | 47% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | additional information | no inhibition by EDTA, DTT, iodoacetic acid, and o-phenanthroline | Pseudomonas aeruginosa | |
3.1.1.3 | PMSF | 19% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | SDS | 93% inhibition at 5 mM | Pseudomonas aeruginosa | |
3.1.1.3 | Zn2+ | 85% inhibition at 5 mM | Pseudomonas aeruginosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | extracellular | the enzyme is secreted to the culture medium | Pseudomonas aeruginosa | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 54000 | - |
x * 54000, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | triacylglycerol + H2O | Pseudomonas aeruginosa | - |
diacylglycerol + a carboxylate | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.3 | Acetone | at 25% v/v, 30°C, 95% remaining activity after 24 h, 33% after 48 h | Pseudomonas aeruginosa |
3.1.1.3 | Butanol | at 25% v/v, 30°C, 10% remaining activity after 48 h | Pseudomonas aeruginosa |
3.1.1.3 | chloroform | at 25% v/v, 30°C, 130% activity after 24 h, 140% after 48 h | Pseudomonas aeruginosa |
3.1.1.3 | dimethylformamide | at 25% v/v, 30°C, 120% activity after 24 h, 110% after 48 h, 100% remaining activity after 30 min at 50°C | Pseudomonas aeruginosa |
3.1.1.3 | Ethanol | at 25% v/v, 30°C, 90% remaining activity after 24 h, 17% after 48 h, 55% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
3.1.1.3 | ethyl methyl ketone | 75% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
3.1.1.3 | hexane | at 25% v/v, 30°C, 100% remaining activity after 24 h, 64% after 48 h, 100% reaming activity after 15 min at 60°C, 50% reamining activity after 30 min at 60°C | Pseudomonas aeruginosa |
3.1.1.3 | isopropanol | at 25% v/v, 30°C, 65% remaining activity after 24 h, 16% after 48 h | Pseudomonas aeruginosa |
3.1.1.3 | Methanol | at 25% v/v, 30°C, 80% remaining activity after 24 h, 42% after 48 h, 100% remaining activity after 10 min at 50°C | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Pseudomonas aeruginosa | - |
strain san-ai, isolated from putrid mineral cutting oil, with pH 10.0, as component of metalworking fluid for cooling and lubrication in industrial metalworking processes | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | native enzyme 12.5fold from culture broth by ammonium sulfate fractionation, hydrophobic interaction chromatography, dialysis, and gel filtration to homogeneity | Pseudomonas aeruginosa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 0.00006 | - |
purified enzyme, 95% acetone, 30°C | Pseudomonas aeruginosa |
3.1.1.3 | 0.00012 | - |
purified enzyme, 95% ethyl-methyl-ketone, 30°C | Pseudomonas aeruginosa |
3.1.1.3 | 0.00013 | - |
purified enzyme, 95% n-hexane, 30°C | Pseudomonas aeruginosa |
3.1.1.3 | 0.0052 | - |
purified enzyme, 95% ethanol, 30°C | Pseudomonas aeruginosa |
3.1.1.3 | 0.231 | - |
purified enzyme, 95% methanol, 30°C | Pseudomonas aeruginosa |
3.1.1.3 | 473 | - |
purified enzyme, buffer | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | methyl oleate + H2O | acylglycerol lipase activity, EC 3.1.1.23 | Pseudomonas aeruginosa | methanol + oleate | - |
? | |
3.1.1.3 | triacylglycerol + H2O | - |
Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
3.1.1.3 | triacylglycerol + H2O | the enzyme acts on triacylglycerols with medium fatty acid chain length, substrate specificity, overview | Pseudomonas aeruginosa | diacylglycerol + a carboxylate | - |
? | |
3.1.1.3 | tributyrin + H2O | - |
Pseudomonas aeruginosa | dibutyrin + butyrate | - |
? | |
3.1.1.3 | tricaprin + H2O | high activity | Pseudomonas aeruginosa | dicaprin + caprate | - |
? | |
3.1.1.3 | tricaproin + H2O | high activity | Pseudomonas aeruginosa | dicaproin + caproate | - |
? | |
3.1.1.3 | tricaprylin + H2O | best substrate | Pseudomonas aeruginosa | dicaprylin + caprylate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Pseudomonas aeruginosa | diolein + oleate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 54000, SDS-PAGE | Pseudomonas aeruginosa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | alkaline lipase | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 70 | - |
- |
Pseudomonas aeruginosa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 30 | 90 | - |
Pseudomonas aeruginosa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 60 | - |
15 min, purified native enzyme, 75% remaining activity | Pseudomonas aeruginosa |
3.1.1.3 | 70 | - |
15 min, purified native enzyme, 30% remaining activity | Pseudomonas aeruginosa |
3.1.1.3 | 80 | - |
complete inactivation within 10 min | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 11 | - |
- |
Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 4 | 11.5 | activity range | Pseudomonas aeruginosa |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 4 | 11.5 | stable | Pseudomonas aeruginosa |