Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kreimeyer, A.; Perret, A.; Lechaplais, C.; Vallenet, D.; Medigue, C.; Salanoubat, M.; Weissenbach, J.
    Identification of the last unknown genes in the fermentation pathway of lysine (2007), J. Biol. Chem., 282, 7191-7197.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.14 biotechnology lysine fermentation pathway Anaerobic bacteria

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.247 gene kce, DNA and amino acid sequence determination and analysis, genetic structure and comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 DE3 Fusobacterium nucleatum subsp. nucleatum
4.3.1.14 in Escherichia coli Anaerobic bacteria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.14 55
-
crotonyl-CoA
-
Anaerobic bacteria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.247 30000
-
4 * 30000, SDS-PAGE, 4 * 31044, sequence calculation Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 31044
-
4 * 30000, SDS-PAGE, 4 * 31044, sequence calculation Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 120000
-
about, gel filtration Fusobacterium nucleatum subsp. nucleatum
4.3.1.14 17000
-
SDS-PAGE Anaerobic bacteria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum
-
L-3-aminobutanoyl-CoA + acetoacetate
-
r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847
-
L-3-aminobutanoyl-CoA + acetoacetate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.247 Fusobacterium nucleatum subsp. nucleatum Q8RHX2 gene kce or FN1868
-
2.3.1.247 Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 Q8RHX2 gene kce or FN1868
-
4.3.1.14 Anaerobic bacteria
-
environmental sample obtained from anaerobic digesters, putative lysine-fermenting bacteria like Fusobacterium nucleatum subsp. nucleatum, Fusobacterium nucleatum subsp. vincentii, Thermoanaerobacter tengcongensis, Porphyromonas gingivalis, Alkaliphilus metalliredigenes, Myxococcus xanthus, Anaeromyxobacter dehlogenans, Clostridium sp., Rhodoferax ferrireducens, Symbiobacterium thermophilum, Nocarioides, Janibacter sp.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.247 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, and gel filtration Fusobacterium nucleatum subsp. nucleatum
4.3.1.14 of the recombinant proteins by HiPrep 26/10 desalting column chromatography Anaerobic bacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
Fusobacterium nucleatum subsp. nucleatum L-3-aminobutanoyl-CoA + acetoacetate
-
r
2.3.1.247 (5S)-5-amino-3-oxohexanoate + acetyl-CoA
-
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / JCM14847 L-3-aminobutanoyl-CoA + acetoacetate
-
r
4.3.1.14 L-3-Aminobutanoyl-CoA
-
Anaerobic bacteria Crotonyl-CoA + NH3
-
r

Subunits

EC Number Subunits Comment Organism
2.3.1.247 homotetramer 4 * 30000, SDS-PAGE, 4 * 31044, sequence calculation Fusobacterium nucleatum subsp. nucleatum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.247 FN1868
-
Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 kce
-
Fusobacterium nucleatum subsp. nucleatum
4.3.1.14 3-aminobutyryl-CoA ammonia lyase
-
Anaerobic bacteria
4.3.1.14 Kal
-
Anaerobic bacteria

General Information

EC Number General Information Comment Organism
2.3.1.247 evolution gene cluster organization in putative lysine-fermenting bacteria, overview Fusobacterium nucleatum subsp. nucleatum
2.3.1.247 metabolism the enzyme is involved in the fermentation pathway of lysine Fusobacterium nucleatum subsp. nucleatum