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Literature summary extracted from

  • Ohnishi, T.; Ohba, H.; Seo, K.C.; Im, J.; Sato, Y.; Iwayama, Y.; Furuichi, T.; Chung, S.K.; Yoshikawa, T.
    Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1 (2007), J. Biol. Chem., 282, 637-646.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in HEK-293T cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.25 D104N IMPA2 mutant, no activity Mus musculus
3.1.3.25 D93N IMPA1 mutant, no activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.25 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Mg2+ required Mus musculus
3.1.3.25 additional information the enzyme shows weaker or almost no activity when Mg2+ is replaced by Ca2+, Mn2+, Na+, or K+ (2 mM each) Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 70000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Mus musculus O55023 IMPA1
-
3.1.3.25 Mus musculus Q91UZ5 IMPA2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.25 brain
-
 Mus musculus
-
3.1.3.25 brain cortex
-
 Mus musculus
-
3.1.3.25 cerebellum
-
 Mus musculus
-
3.1.3.25 heart
-
 Mus musculus
-
3.1.3.25 hippocampus
-
 Mus musculus
-
3.1.3.25 kidney
-
 Mus musculus
-
3.1.3.25 liver
-
 Mus musculus
-
3.1.3.25 olfactory bulb
-
 Mus musculus
-
3.1.3.25 pancreas
-
 Mus musculus
-
3.1.3.25 small intestine
-
 Mus musculus
-
3.1.3.25 spleen
-
 Mus musculus
-
3.1.3.25 testis
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 alpha-D-glucose 1-phosphate IMPA1 shows intermediate activity towards alpha-D-glucose 1-phosphate, whereas IMPA2 shows significantly lower affinity Mus musculus alpha-D-glucose + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O IMPA1 shows strong activity towards D-myo-inositol 1-phosphate, whereas IMPA2 shows significantly lower affinity Mus musculus D-myo-inositol + phosphate
-
 ?
3.1.3.25 scyllo-inositol 1-phosphate + H2O IMPA1 shows strong activity towards scyllo-inositol 1-phosphate, whereas IMPA2 shows almost no affinity Mus musculus scyllo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 homodimer x-ray crystallography Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPA1
-
 Mus musculus
3.1.3.25 IMPA2
-
 Mus musculus
3.1.3.25 IMPase
-
 Mus musculus
3.1.3.25 myo-inositol monophosphatase
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 7 7.5 IMPA1 Mus musculus
3.1.3.25 7.5 8 IMPA2 Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.25 8
-
 the activity of IMPA1 drops to 30% at pH 8.0 Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.25 0.7
-
 IMPA1, in the presence of 2 mM MgCl2 Mus musculus Li+
3.1.3.25 25
-
 IMPA2, in the presence of 2 mM MgCl2 Mus musculus Li+