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Literature summary extracted from

  • de Eugenio, L.I.; Garcia, P.; Luengo, J.M.; Sanz, J.M.; Roman, J.S.; Garcia, J.L.; Prieto, M.A.
    Biochemical evidence that phaZ gene encodes a specific intracellular medium chain length polyhydroxyalkanoate depolymerase in Pseudomonas putida KT2442: characterization of a paradigmatic enzyme (2007), J. Biol. Chem., 282, 4951-4962.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 overexpression in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 dithiothreitol
-
 Pseudomonas putida
3.1.1.76 mercaptoethanol
-
 Pseudomonas putida
3.1.1.76 phenylmethylsulfonyl fluoride
-
 Pseudomonas putida
3.1.1.76 SDS
-
 Pseudomonas putida
3.1.1.76 sodium cholate
-
 Pseudomonas putida
3.1.1.76 Triton X-100
-
 Pseudomonas putida
3.1.1.76 Tween-20
-
 Pseudomonas putida
3.1.1.76 Tween-80
-
 Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 intracellular
-
 Pseudomonas putida 5622
-
3.1.1.76 PHA granule
-
 Pseudomonas putida 70088
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.76 additional information for optimal activity, it is important to maintain a high ionic strength (300mM NaCl) in the reaction mixture Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas putida Q5Y152
-
-
3.1.1.76 Pseudomonas putida KT 2442 Q5Y152
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 1,2,3-tributyryl-sn-glycerol + H2O
-
 Pseudomonas putida ?
-
 ?
3.1.1.76 1,2,3-tributyryl-sn-glycerol + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?
3.1.1.76 additional information hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) Pseudomonas putida ?
-
 ?
3.1.1.76 additional information hydrolyzes specifically mcl-PHAs containing aliphatic and aromatic monomers, e.g. P(HO-co-HX)n (HO: 87%, HX: 13%), P(HO-co-HX)s (HO: 87%, HX: 13%), PHPhh, PHPhO, P(HN-co-HP) (HN: 55%, HP: 45%) Pseudomonas putida KT 2442 ?
-
 ?
3.1.1.76 poly(3-hydroxyphenylheptanoate) + H2O
-
 Pseudomonas putida ?
-
 ?
3.1.1.76 poly(3-hydroxyphenylheptanoate) + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?
3.1.1.76 poly(3-hydroxyphenyloctanoate) + H2O
-
 Pseudomonas putida ?
-
 ?
3.1.1.76 poly(3-hydroxyphenyloctanoate) + H2O
-
 Pseudomonas putida KT 2442 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 medium chain length polyhydroxyalkanoate depolymerase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 43
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.76 37
-
 very unstable above Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8.8
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.76 7 10.5 pH 7.0: about 25% of maximal activity, pH 10.5: about 50% of maximal activity Pseudomonas putida