BRENDA - Enzyme Database

Plastidic phosphatidic acid phosphatases identified in a distinct subfamily of lipid phosphate phosphatases with prokaryotic origin

Nakamura, Y.; Tsuchiya, M.; Ohta, H.; J. Biol. Chem. 282, 29013-29021 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
isozyme LPPbeta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree; isozyme LPPepsilon1, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation; isozyme LPPepsilon2, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic ARabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation; isozyme LPPgamma, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of isozyme LPPgamma in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation; isozymes LPPdelta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
a lppepsilon1lppepsilon2 double knock-out mutant shows no significant changes in lipid composition; a lppepsilon1lppepsilon2 double knock-out mutant shows no significant changes in lipid composition; a lppgamma homozygous mutant is isolated only under ectopic overexpression of LPPgamma, suggesting that loss of LPPgamma may cause lethal effect on plant viability
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Mg2+
about 75% inhibition at 20 mM, isozyme LPPgamma; about 95% inhibition at 20 mM, isozyme LPPepsilon1
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
chloroplast
the homolog of cyanobacterial LPP, isozyme LPPepsilon1, is localized to chloroplasts and contains a plastidic transit peptide; the homolog of cyanobacterial LPP, isozyme LPPepsilon2, is localized to chloroplasts and contains a plastidic transit peptide; the homolog of cyanobacterial LPP, isozyme LPPgamma, is localized to chloroplasts and contains a plastidic transit peptide
Arabidopsis thaliana
9507
-
3.1.3.4
additional information
isozyme LPPbeta contains no plastidic transit peptide; isozyme LPPdelta contains no plastidic transit peptide
Arabidopsis thaliana
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
50% inhibition at 10-20 mM, isozyme LPPepsilon2
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
23371
-
x * 23371, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
3.1.3.4
25736
-
x * 25736, isozyme LPPgamma, sequence calculation
Arabidopsis thaliana
3.1.3.4
30578
-
x * 30578, isozyme LPPepsilon1, sequence calculation
Arabidopsis thaliana
3.1.3.4
31512
-
x * 31512, isozyme LPPepsilon2, sequence calculation
Arabidopsis thaliana
3.1.3.4
46151
-
x * 46151, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
phosphatidic acid + H2O
Arabidopsis thaliana
-
1,2-sn-diacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Arabidopsis thaliana
the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes
1,2-sn-diacylglycerol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Arabidopsis thaliana
-
This UniProt-ID has been deleted; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
3.1.3.4
Arabidopsis thaliana
Q0WUC2
isozyme LPPdelta; ecotype Columbia-0
-
3.1.3.4
Arabidopsis thaliana
Q6NLA5
isozyme LPPgamma; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
3.1.3.4
Arabidopsis thaliana
Q6NQL6
isozyme LPPepsilon2; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
3.1.3.4
Arabidopsis thaliana
Q9SUW4
isozyme LPPbeta; ecotype Columbia-0, Arabidopsis contains five homologs of cyanobacterial LPP
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
chloroplast preparation from native and transgenic plants; chloroplast preparation from native and transgenic plants; chloroplast preparation from native and transgenic plants
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
leaf
-
Arabidopsis thaliana
-
3.1.3.4
shoot
high expression of isozyme LPPgamma
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
680823
Arabidopsis thaliana
1,2-sn-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes
680823
Arabidopsis thaliana
1,2-sn-diacylglycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 23371, isozyme LPPdelta, sequence calculation; x * 25736, isozyme LPPgamma, sequence calculation; x * 30578, isozyme LPPepsilon1, sequence calculation; x * 31512, isozyme LPPepsilon2, sequence calculation; x * 46151, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
8
isozyme LPPgamma, broad optimum
Arabidopsis thaliana
3.1.3.4
7
-
isozyme LPPepsilon1; isozyme LPPepsilon2
Arabidopsis thaliana
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
5
10
isozyme LPPepsilon1; isozyme LPPepsilon2; isozyme LPPgamma
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
isozyme LPPbeta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree
Arabidopsis thaliana
3.1.3.4
isozyme LPPepsilon1, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
Arabidopsis thaliana
3.1.3.4
isozyme LPPepsilon2, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of the isozyme in transgenic ARabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
Arabidopsis thaliana
3.1.3.4
isozyme LPPgamma, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree, functional complementation of a PAP-deficient yeast DELTAdpp1DELTAlpp1DELTApah1 by the plastidic LPP, phenotype rescue in vivo and in vitro, overview, expression of isozyme LPPgamma in transgenic Arabidopsis thaliana plants using Agrobacterium tumefaciens-mediated transformation
Arabidopsis thaliana
3.1.3.4
isozymes LPPdelta, DNA and amino acid sequence determination and analysis, LPP subfamily phylogenetic tree
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
a lppgamma homozygous mutant is isolated only under ectopic overexpression of LPPgamma, suggesting that loss of LPPgamma may cause lethal effect on plant viability
Arabidopsis thaliana
3.1.3.4
additional information
a lppepsilon1lppepsilon2 double knock-out mutant shows no significant changes in lipid composition
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Mg2+
about 75% inhibition at 20 mM, isozyme LPPgamma
Arabidopsis thaliana
3.1.3.4
Mg2+
about 95% inhibition at 20 mM, isozyme LPPepsilon1
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
chloroplast
the homolog of cyanobacterial LPP, isozyme LPPgamma, is localized to chloroplasts and contains a plastidic transit peptide
Arabidopsis thaliana
9507
-
3.1.3.4
chloroplast
the homolog of cyanobacterial LPP, isozyme LPPepsilon2, is localized to chloroplasts and contains a plastidic transit peptide
Arabidopsis thaliana
9507
-
3.1.3.4
chloroplast
the homolog of cyanobacterial LPP, isozyme LPPepsilon1, is localized to chloroplasts and contains a plastidic transit peptide
Arabidopsis thaliana
9507
-
3.1.3.4
additional information
isozyme LPPbeta contains no plastidic transit peptide
Arabidopsis thaliana
-
-
3.1.3.4
additional information
isozyme LPPdelta contains no plastidic transit peptide
Arabidopsis thaliana
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
50% inhibition at 10-20 mM, isozyme LPPepsilon2
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
23371
-
x * 23371, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
3.1.3.4
25736
-
x * 25736, isozyme LPPgamma, sequence calculation
Arabidopsis thaliana
3.1.3.4
30578
-
x * 30578, isozyme LPPepsilon1, sequence calculation
Arabidopsis thaliana
3.1.3.4
31512
-
x * 31512, isozyme LPPepsilon2, sequence calculation
Arabidopsis thaliana
3.1.3.4
46151
-
x * 46151, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
phosphatidic acid + H2O
Arabidopsis thaliana
-
1,2-sn-diacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Arabidopsis thaliana
the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes
1,2-sn-diacylglycerol + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
chloroplast preparation from native and transgenic plants
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
leaf
-
Arabidopsis thaliana
-
3.1.3.4
shoot
high expression of isozyme LPPgamma
Arabidopsis thaliana
-
3.1.3.4
shoot
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
phosphatidic acid + H2O
-
680823
Arabidopsis thaliana
1,2-sn-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
the plastidic phosphatidic acid phosphatase dephosphorylates phosphatidic acid to yield diacylglycerol, which is a precursor for galactolipids, a primary and indispensable component of photosynthetic membranes
680823
Arabidopsis thaliana
1,2-sn-diacylglycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 23371, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
3.1.3.4
?
x * 25736, isozyme LPPgamma, sequence calculation
Arabidopsis thaliana
3.1.3.4
?
x * 46151, isozyme LPPdelta, sequence calculation
Arabidopsis thaliana
3.1.3.4
?
x * 31512, isozyme LPPepsilon2, sequence calculation
Arabidopsis thaliana
3.1.3.4
?
x * 30578, isozyme LPPepsilon1, sequence calculation
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
8
isozyme LPPgamma, broad optimum
Arabidopsis thaliana
3.1.3.4
7
-
isozyme LPPepsilon2
Arabidopsis thaliana
3.1.3.4
7
-
isozyme LPPepsilon1
Arabidopsis thaliana
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
5
10
isozyme LPPgamma
Arabidopsis thaliana
3.1.3.4
5
10
isozyme LPPepsilon2
Arabidopsis thaliana
3.1.3.4
5
10
isozyme LPPepsilon1
Arabidopsis thaliana