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Literature summary extracted from

  • Moeker, N.; Reihlen, P.; Kraemer, R.; Morbach, S.
    Osmosensing properties of the histidine protein kinase MtrB from Corynebacterium glutamicum (2007), J. Biol. Chem., 282, 27666-27677.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.13.3 alanine stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 betaine stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 fructose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 glucose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 glutamate stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 L-glutamate stimulates, more efficient stimulation of MtrB than MtrA Corynebacterium glutamicum
2.7.13.3 lysine stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 maltoheptaose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 maltose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 maltotriose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 additional information liposome shrinkage is not necessary for MtrB activation, MtrB is activated upon the addition of various chemical compounds, like sugars, amino acids, and polyethylene glycols, these may act via a change of the hydration state of the protein shifting MtrB into the active state, for activation it is essential that solutes are added at the same side as the cytoplasmic domains of the kinase are located Corynebacterium glutamicum
2.7.13.3 NaCl provides minor activation Corynebacterium glutamicum
2.7.13.3 proline stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 sucrose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum
2.7.13.3 trehalose stimulates, stimulation pattern of MtrA is similar to that of MtrB Corynebacterium glutamicum

Application

EC Number Application Comment Organism
2.7.13.3 additional information MtrB belongs to a class of histidine protein kinases that sense environmental changes at cytoplasmatic protein domains independently of the periplasmic loop and the cytoplasmic HAMP domain Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 Escherichia coli BL21(DE3) cells transformed with different pASK-IBA7-mtrB strep derivatives Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 additional information in the deltamtrAB mutant, the expression of carrier genes is abolished (betP) or markedly reduced, MtrB mutants in which either the large periplasmic loop or the HAMP domain is deleted, are regulated similar to wild-type MtrB Corynebacterium glutamicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.13.3 membrane
-
 Corynebacterium glutamicum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.13.3 54000
-
 x * 54000, SDS-PAGE of MtrB Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Corynebacterium glutamicum
-
 strain ATCC 13032
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.13.3 by gel filtration Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
2.7.13.3 -80°C, 50 mM Tris/HCl buffer, pH 8.0, 500 mM NaCl, 10% glycerol, 10 mM beta-mercaptoethanol Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 additional information autophosphorylation of MtrB-Strep in proteoliposomes in the presence of ATP Corynebacterium glutamicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.13.3 ? x * 54000, SDS-PAGE of MtrB Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 histidine protein kinase
-
 Corynebacterium glutamicum
2.7.13.3 MtrA
-
 Corynebacterium glutamicum
2.7.13.3 MtrB
-
 Corynebacterium glutamicum