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Literature summary extracted from

  • Hatori, Y.; Majima, E.; Tsuda, T.; Toyoshima, C.
    Domain organization and movements in heavy metal ion pumps: papain digestion of CopA, a Cu+-transporting ATPase (2007), J. Biol. Chem., 282, 25213-25221.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.8 expressed in Escherichia coli DH5alpha cells Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.8 C12E8
-
 Thermotoga maritima
7.2.2.8 additional information ATPase activity os almost completely suppressed by incubating CopA (1 mg/ml or 0.00633 mM) for 1 h in a solution containing 1 mM MgCl2, 0.1 mM AlCl3, 2 mM potassium fluoride in the presence of either 0.02 mM Cu+,1 mM ADP, and 0.02mM Cu+, or 0.5 mM bathocuproine disulfonate Thermotoga maritima
7.2.2.8 N,N-Dimethyldodecylamine-N-oxide
-
 Thermotoga maritima
7.2.2.8 n-octyl beta-D-glucopyranoside
-
 Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.8 0.0031
-
 Cu+[side 1] at pH 6.0 and 60°C Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.8 microsome
-
 Thermotoga maritima
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.8 Cu+ 100% activity at 0.02 mM Cu+ Thermotoga maritima
7.2.2.8 Mg2+ 100% activity at 1 mM Mg2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.8 83300
-
 x * 83300, SELDI-TOF mass spectrometry Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1] Thermotoga maritima
-
 ADP + phosphate + Cu+[side 2]
-
 ?
7.2.2.8 additional information Thermotoga maritima the enzyme also shows Ag+-dependent ATPase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-
7.2.2.8 Thermotoga maritima Q9WYF3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.8 Co2+ metal affinity resin column chromatography, and gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 CopA + H2O CopA is a bacterial Cu+-ATPase from Thermotoga maritima and contains 3 papain cleavage sites on the C-terminal side of the N-terminal metal binding domain Carica papaya ?
-
 ?
3.4.22.2 N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
 Carica papaya N-alpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.2 sarcoendoplasmic reticulum Ca2+-ATPase 1 + H2O
-
 Carica papaya ?
-
 ?
7.2.2.8 ATP + H2O + Cu+[side 1]
-
 Thermotoga maritima ADP + phosphate + Cu+[side 2]
-
 ?
7.2.2.8 additional information the enzyme also shows Ag+-dependent ATPase activity Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.8 ? x * 83300, SELDI-TOF mass spectrometry Thermotoga maritima
7.2.2.8 ? x * 83400, calculated from amino acid sequence Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 CopA
-
 Thermotoga maritima
7.2.2.8 Cu+-ATPase
-
 Thermotoga maritima
7.2.2.8 TM0317
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.2.2.8 60
-
 Cu+-ATPase activity reaches the maximum at 60°C and is 4.4% of the maximum at 30°C Thermotoga maritima