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Literature summary extracted from

  • Kreel, N.E.; Tabita, F.R.
    Substitutions at methionine 295 of Archaeoglobus fulgidus ribulose-1,5-bisphosphate carboxylase/oxygenase affect oxygen binding and CO2/O2 specificity (2007), J. Biol. Chem., 282, 1341-1351.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.39 expressed in Escherichia coli strain BL21 Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 M295D less sensitive to oxygen compared with the wild-type enzyme, retains 35-40% of activity after exposing to O2 Archaeoglobus fulgidus
4.1.1.39 M295D/S363I less sensitive to oxygen compared with the wild-type enzyme, retains 82% of activity after exposing to O2 Archaeoglobus fulgidus
4.1.1.39 M295D/S363V less sensitive to oxygen compared with the wild-type enzyme, retains 86% of activity after exposing to O2 Archaeoglobus fulgidus
4.1.1.39 S363I less sensitive to oxygen compared with the wild-type enzyme Archaeoglobus fulgidus
4.1.1.39 S363V less sensitive to oxygen compared with the wild-type enzyme Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.39 O2 competitive inhibitor with respect to CO2, assay in the presence of oxygen and high levels of CO2 results in substantial loss (85-90%) of activity compared with assays performed under strictly anaerobic conditions Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.39 0.005
-
 O2 wild type enzyme, at 83°C Archaeoglobus fulgidus
4.1.1.39 0.0205
-
 D-ribulose 1,5-bisphosphate wild type enzyme, at 83°C Archaeoglobus fulgidus
4.1.1.39 0.021
-
 D-ribulose 1,5-bisphosphate mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
4.1.1.39 0.024
-
 O2 mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
4.1.1.39 0.051
-
 CO2 wild type enzyme, at 83°C Archaeoglobus fulgidus
4.1.1.39 0.058
-
 CO2 mutant enzyme M295D, at 83°C Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.39 48500
-
 2 * 48500, SDS-PAGE Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 Q-Sepharose column chromatography, Superose 12 gel filtration, and phenyl-Sepharose column chromatography Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Archaeoglobus fulgidus 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + O2
-
 Archaeoglobus fulgidus 3-phospho-D-glycerate + 2-phosphoglycolate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.39 homodimer 2 * 48500, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 form III ribulose-1,5-bisphosphate carboxylase/oxygenase
-
 Archaeoglobus fulgidus
4.1.1.39 RbcL2
-
 Archaeoglobus fulgidus
4.1.1.39 Rubisco
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.39 83
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.39 23 93
-
 Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.39 17.7
-
 D-ribulose 1,5-bisphosphate mutant enzyme M295D, at 83°C Archaeoglobus fulgidus
4.1.1.39 23.1
-
 D-ribulose 1,5-bisphosphate wild type enzyme, at 83°C Archaeoglobus fulgidus