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Literature summary extracted from

  • Zeidan, Y.H.; Hannun, Y.A.
    Activation of acid sphingomyelinase by protein kinase Cdelta-mediated phosphorylation (2007), J. Biol. Chem., 282, 11549-11561.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 phorbol 12-myristate 13-acetate/protein kinase Cdelta activation of acid sphingomyelinase by protein kinase Cdelta-mediated phosphorylation, which is mediated by phorbol 12-myristate 13-acetate, that induces also membrane translocation of ASMase, formation of a novel PKCdelta-ASMase complex after PMA stimulation, mechanism, overview Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 gene SMPD1, transient overexpression of wild-type ASMase and mutant S508A in MCF-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 additional information silencing of ASMase by siRNA Homo sapiens
3.1.4.12 S508A site-directed mutagenesis of ASMase, the mutation blocks the ceramide formation after phorbol 12-myristate 13-acetate treatment, the mutant acts dominantly negative Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 lysosomal membrane translocation of ASMase from endolysosomes to the plasma membrane after sustained phorbol 12-myristate 13-acetate treatment Homo sapiens 5765
-
3.1.4.12 plasma membrane translocation of ASMase from endolysosomes to the plasma membrane after sustained phorbol 12-myristate 13-acetate treatment Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Homo sapiens ASMase is required for phorbol 12-myristate 13-acetate-induced ceramide formation involving protein kinase Cdelta, overview N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
 gene SMPD1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.12 phosphoprotein acid sphingomyelinase is activated by protein kinase Cdelta phosphorylation at Ser508, which is mediated by phorbol 12-myristate 13-acetate, overview Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 MCF-7 cell mammary carcinoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O ASMase is required for phorbol 12-myristate 13-acetate-induced ceramide formation involving protein kinase Cdelta, overview Homo sapiens N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 acid sphingomyelinase
-
 Homo sapiens
3.1.4.12 aSMase
-
 Homo sapiens