Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishibashi, Y.; Nakasone, T.; Kiyohara, M.; Horibata, Y.; Sakaguchi, K.; Hijikata, A.; Ichinose, S.; Omori, A.; Yasui, Y.; Imamura, A.; Ishida, H.; Kiso, M.; Okino, N.; Ito, M.
    A novel endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides (2007), J. Biol. Chem., 282, 11386-11396.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.123 amplified gene product encoding amino acid residues 23-488 without the signal peptide digested with HindIII and NotI and inserted into the corresponding sites of pET22b(+) to generate a C-terminal His6-tagged protein. The recombinant plasmid and mutants expressed in Escherichia coli strain BL21(DE3) Rhodococcus equi

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.123 E234D strongly decreased enzyme activity compared with the wild-type enzyme Rhodococcus equi
3.2.1.123 E234Q no activity Rhodococcus equi
3.2.1.123 E341D strongly decreased enzyme activity compared with the wild-type enzyme Rhodococcus equi
3.2.1.123 E341Q no activity Rhodococcus equi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.123 Cu2+
-
 Rhodococcus equi
3.2.1.123 Fe3+
-
 Rhodococcus equi
3.2.1.123 Hg2+ completely inhibits enzyme activity Rhodococcus equi
3.2.1.123 additional information Ca2+, Mg2+, Mn2+, Ni2+, Co2+, and EDTA have no significant effect on the activity Rhodococcus equi
3.2.1.123 taurodeoxycholate strongly inhibits the enzyme activity at a concentration of 0.05% Rhodococcus equi
3.2.1.123 Zn2+
-
 Rhodococcus equi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.123 0.43
-
 Galbeta1-6Galbeta1-6Galbeta1-1'ceramide
-
 Rhodococcus equi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.123 52299
-
 x * 54000, SDS-PAGE, x * 52299, sequence analysis Rhodococcus equi
3.2.1.123 54000
-
 x * 54000, SDS-PAGE, x * 52299, sequence analysis Rhodococcus equi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.123 Rhodococcus equi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.123 by gel filtration, 2100fold Rhodococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.123 Galalpha1-6Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O most rapidly hydrolyzed Rhodococcus equi ceramide + Galalpha1-6Galalpha1-6Gal
-
 ?
3.2.1.123 Galalpha1-6Galbeta1-6Galbeta1-1'ceramide + H2O
-
 Rhodococcus equi ceramide + Galalpha1-6Galbeta1-6Gal
-
 ?
3.2.1.123 Galbeta1-6Galbeta1-1'ceramide + H2O
-
 Rhodococcus equi ceramide + Galbeta1-6Galbeta
-
 ?
3.2.1.123 Galbeta1-6Galbeta1-6Galbeta1-1'-ceramide + H2O
-
 Rhodococcus equi ceramide + Galbeta1-6Galbeta1-6Gal
-
 ?
3.2.1.123 monogalactosylceramide + H2O
-
 Rhodococcus equi ceramide + D-galactose
-
 ?
3.2.1.123 additional information does not hydrolyze ganglio-, globo-, or lacto-series glycosphingolipids Rhodococcus equi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.123 ? x * 54000, SDS-PAGE, x * 52299, sequence analysis Rhodococcus equi

Synonyms

EC Number Synonyms Comment Organism
3.2.1.123 ceramide glycanase
-
 Rhodococcus equi
3.2.1.123 EGALC
-
 Rhodococcus equi
3.2.1.123 endogalactosylceramidase
-
 Rhodococcus equi
3.2.1.123 endoglycoceramidase
-
 Rhodococcus equi
3.2.1.123 oligogalactosyl-N-acylsphingosine 1,1'-beta-galactohydrolase
-
 Rhodococcus equi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.123 13.3
-
 Galbeta1-6Galbeta1-6Galbeta1-1'ceramide
-
 Rhodococcus equi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.123 5.5
-
-
 Rhodococcus equi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.123 Rhodococcus equi sequence analysis
-
 4.28