Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Meray, R.K.; Lansbury, P.T.
    Reversible monoubiquitination regulates the Parkinson disease-associated ubiquitin hydrolase UCH-L1 (2007), J. Biol. Chem., 282, 10567-10575.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.12 medicine Parkinson disease-associated UCH-L1 is regulated by reversible monoubiquitination involving auto-deubiquitination Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.12 COS-7 cells co-transfected with plasmids encoding His-tagged UCH-L1 and FLAG-tagged ubiquitin Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.19.12 C90S increases cellular monoubiquitin levels, 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated Homo sapiens
3.4.19.12 C90S/K157R reduces monoubiquitination Homo sapiens
3.4.19.12 D30K deficient in ubiquitin binding, is unable to increase cellular monoubiquitin levels Homo sapiens
3.4.19.12 H161D 30% is monoubiquitinated, whereas only 5-10% of wild-type is monoubiquitinated Homo sapiens
3.4.19.12 I93M approximately doubles the monoubiquitination level of UCH-L1 Homo sapiens
3.4.19.12 additional information fusion protein UbVV/L8A-UCH-L1 contains an L8A mutation in the ubiquitin moiety. Efficiently binds ubiquitin, increases cellular monoubiquitin levels Homo sapiens
3.4.19.12 S18Y does not affect cellular levels of monoubiquitinated UCH-L1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.12 iodoacetamide prevents deubiquitination Homo sapiens
3.4.19.12 additional information monoubiquitination inhibits ubiquitin binding in vitro and prevents UCH-L1 from regulating free ubiquitin levels in cells involving auto-deubiquitination. Intramolecular deubiquitination, which is dependent on the hydrolytic activity of UCH-L1, regulates the lifetime of monoubiquitination, thereby affecting the cellular level of monoubiquitinated UCH-L1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.12 cytoplasm
-
 Homo sapiens 5737
-
3.4.19.12 additional information perinucleus Homo sapiens
-
-
3.4.19.12 nucleus
-
 Homo sapiens 5634
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.12 on Ni-NTA resin Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.12 ubiquitin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.12 ubiquitin C-terminal hydrolase-L1
-
 Homo sapiens
3.4.19.12 UCH-L1
-
 Homo sapiens