BRENDA - Enzyme Database

Lipid phosphate phosphatase-2 activity regulates S-phase entry of the cell cycle in Rat2 fibroblasts

Morris, K.E.; Schang, L.M.; Brindley, D.N.; J. Biol. Chem. 281, 9297-9306 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
3.1.3.4
additional information
increasing LPP2 activity causes premature entry into S-phase, premature cyclin A expression and decreased rates of proliferation at high passage and accumulation in G2/M. Cells transduced with LPP2 activate the G2/M checkpoint and show characteristics of senescence at high passage
Homo sapiens
3.1.3.4
additional information
fibroblasts that overexpress LPP1 enter S-phase at approximately the same time as vector control cells
Mus musculus
3.1.3.4
additional information
fibroblasts that overexpress LPP3 enter S-phase at approximately the same time as vector control cells
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
functional overexpression of GFP-tagged LPPs in rat2 fibroblasts, the transformed cells show reduced proliferation, overview; rat2 fibroblasts transduced and overexpressed with LPP2, LPP2-GFP and mutant LPP2(R214K)-GFP
Homo sapiens
3.1.3.4
rat2 fibroblasts transduced and overexpressed with LPP1 and mLPP1-GFP
Mus musculus
3.1.3.4
rat2 fibroblasts transduced and overexpressed with LPP3-GFP or myc-LPP3
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
knock-down of LPPs in rat2 fibroblasts, phenotype, overview
Homo sapiens
3.1.3.4
R214K
knock-down of endogenous LPP2 in fibroblasts delays cyclin A accumulation and entry into S-phase of the cell cycle. Cells overexpressing LPP2(R214K)-GFP show no significant change in LPP activity; site-directed mutagenesis
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
siRNA
rat2 fibroblasts treated with siRNA for LPP2 show a ca. 60% decrease in mRNA for the targeted LPP
Homo sapiens
3.1.3.4
siRNA
rat2 fibroblasts treated with siRNA for LPP1 show about a 60% decrease in mRNA for the targeted LPP
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
endoplasmic reticulum
wild-type and mutant
Homo sapiens
5783
-
3.1.3.4
endosome
early stage, wild-type and mutant
Homo sapiens
5768
-
3.1.3.4
membrane
-
Homo sapiens
16020
-
3.1.3.4
additional information
LLP2 is not localized in Golgi apparatus, mitochondria, nucleus, or nuclear membrane; not localized to the Golgi apparatus, mitochondria, nucleus, or nuclear membrane
Homo sapiens
-
-
3.1.3.4
plasma membrane
; wild-type and mutant
Homo sapiens
5886
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
60000
-
immunoprecipitation, band only visible at mRNA overexpression levels of 100fold or more
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
3.1.3.4
Mus musculus
-
-
-
3.1.3.4
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Mus musculus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview
680744
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
680744
Mus musculus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680744
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680744
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Mus musculus
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Homo sapiens
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Rattus norvegicus
sphingosine + phosphate
-
-
-
?
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.4
additional information
increasing LPP2 activity causes premature entry into S-phase, premature cyclin A expression and decreased rates of proliferation at high passage and accumulation in G2/M. Cells transduced with LPP2 activate the G2/M checkpoint and show characteristics of senescence at high passage
Homo sapiens
3.1.3.4
additional information
fibroblasts that overexpress LPP1 enter S-phase at approximately the same time as vector control cells
Mus musculus
3.1.3.4
additional information
fibroblasts that overexpress LPP3 enter S-phase at approximately the same time as vector control cells
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
functional overexpression of GFP-tagged LPPs in rat2 fibroblasts, the transformed cells show reduced proliferation, overview; rat2 fibroblasts transduced and overexpressed with LPP2, LPP2-GFP and mutant LPP2(R214K)-GFP
Homo sapiens
3.1.3.4
rat2 fibroblasts transduced and overexpressed with LPP1 and mLPP1-GFP
Mus musculus
3.1.3.4
rat2 fibroblasts transduced and overexpressed with LPP3-GFP or myc-LPP3
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
knock-down of LPPs in rat2 fibroblasts, phenotype, overview
Homo sapiens
3.1.3.4
R214K
knock-down of endogenous LPP2 in fibroblasts delays cyclin A accumulation and entry into S-phase of the cell cycle. Cells overexpressing LPP2(R214K)-GFP show no significant change in LPP activity; site-directed mutagenesis
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
siRNA
rat2 fibroblasts treated with siRNA for LPP2 show a ca. 60% decrease in mRNA for the targeted LPP
Homo sapiens
3.1.3.4
siRNA
rat2 fibroblasts treated with siRNA for LPP1 show about a 60% decrease in mRNA for the targeted LPP
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
endoplasmic reticulum
wild-type and mutant
Homo sapiens
5783
-
3.1.3.4
endosome
early stage, wild-type and mutant
Homo sapiens
5768
-
3.1.3.4
membrane
-
Homo sapiens
16020
-
3.1.3.4
additional information
LLP2 is not localized in Golgi apparatus, mitochondria, nucleus, or nuclear membrane; not localized to the Golgi apparatus, mitochondria, nucleus, or nuclear membrane
Homo sapiens
-
-
3.1.3.4
plasma membrane
; wild-type and mutant
Homo sapiens
5886
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
60000
-
immunoprecipitation, band only visible at mRNA overexpression levels of 100fold or more
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Homo sapiens
increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Mus musculus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lyso-phosphatidic acid + H2O
-
680744
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
increasing LPP2 activity causes premature cyclin A expression and decreased LPP2 expression delays cyclin A expression, overview
680744
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
680744
Mus musculus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680744
Homo sapiens
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680744
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Mus musculus
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Homo sapiens
sphingosine + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680744
Rattus norvegicus
sphingosine + phosphate
-
-
-
?