EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.19.1 | plasmid containing full-length acylpeptide hydrolase used as template to generate pFLAG-CMV1 expression construct. This construct encodes amino acids 2-732 of acylpeptide hydrolase. For protein production, transfection into 293 cells | synthetic construct |
3.4.21.B28 | expressed in 293 cells | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.19.1 | Ac-Gly-prolineboronic acid | - |
synthetic construct | |
3.4.19.1 | acetyl-Gly-prolineboronic acid | selectivity for APH 25fold higher than for fibroblast activation protein | synthetic construct | |
3.4.21.B28 | acetyl-Gly-Pro-boronic acid | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B28 | 0.106 | - |
Ile-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 0.189 | - |
Lys-Ala-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 0.244 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 0.245 | - |
Phe-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 0.248 | - |
Gly-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.B28 | 97000 | - |
SDS-PAGE | Homo sapiens |
3.4.21.B28 | 100000 | - |
2 * 100000, multiangle light scattering in combination with gel filtration chromatography and interferometric refractometry | Homo sapiens |
3.4.21.B28 | 200000 | - |
multiangle light scattering in combination with gel filtration chromatography and interferometric refractometry | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.19.1 | synthetic construct | - |
- |
- |
3.4.21.B28 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.19.1 | by affinity chromatography, more than 95% pure | synthetic construct |
3.4.21.B28 | M2-anti-FLAG resin column chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.19.1 | acetyl-Ala-7-amido-4-methylcoumarin + H2O | - |
synthetic construct | acetyl-Ala + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | acetyl-Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Ala-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
3.4.21.B28 | Arg-Pro-7-amido-4-methylcoumarin + H2O | preferred substrate | Homo sapiens | Arg-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | benzyloxycarbonyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O | - |
Homo sapiens | benzyloxycarbonyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin | - |
? | |
3.4.21.B28 | biotinyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O | - |
Homo sapiens | biotinyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin | - |
? | |
3.4.21.B28 | Gln-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gln-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Glu-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Glu-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Gly-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
3.4.21.B28 | Ile-Pro-7-amido-4-methylcoumarin + H2O | most preferred substrate | Homo sapiens | Ile-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Ile-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Ile-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
3.4.21.B28 | Leu-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Leu-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Lys-Ala-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Lys-Ala + 7-amino-4-trifluoromethylcoumarin | - |
? | |
3.4.21.B28 | Lys-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Lys-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Met-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Met-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | additional information | shows very weak activity against acetyl-Ser-Pro-7-amido-4-methylcoumarin, Asp-Pro-7-amido-4-methylcoumarin, His-Pro-7-amido-4-methylcoumarin and Asn-Pro-7-amido-4-methylcoumarin, does not cleave succinyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin, Gly-Ala-7-amido-4-methylcoumarin, acetyl-Ala-Pro-7-amido-4-methylcoumarin, acetyl-Asp-Pro-7-amido-4-methylcoumarin, acetyl-Glu-Pro-7-amido-4-methylcoumarin, acetyl-Phe-Pro-7-amido-4-methylcoumarin, acetyl-His-Pro-7-amido-4-methylcoumarin, acetyl-Ile-Pro-7-amido-4-methylcoumarin, acetyl-Lys-Pro-7-amido-4-methylcoumarin, acetyl-Leu-Pro-7-amido-4-methylcoumarin, acetyl-Met-Pro-7-amido-4-methylcoumarin, acetyl-Asn-Pro-7-amido-4-methylcoumarin, acetyl-Pro-Pro-7-amido-4-methylcoumarin, acetyl-Gln-Pro-7-amido-4-methylcoumarin, acetyl-Arg-Pro-7-amido-4-methylcoumarin, acetyl-Thr-Pro-7-amido-4-methylcoumarin, acetyl-Val-Pro-7-amido-4-methylcoumarin, and acetyl-Tyr-Pro-7-amido-4-methylcoumarin | Homo sapiens | ? | - |
? | |
3.4.21.B28 | N-formyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O | - |
Homo sapiens | N-formyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin | - |
? | |
3.4.21.B28 | N-methyl-Gly-Pro-7-amido-4-methyl-3-carbamoylcoumarin + H2O | - |
Homo sapiens | N-methyl-Gly-Pro + 7-amino-4-methyl-3-carbamoylcoumarin | - |
? | |
3.4.21.B28 | Phe-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Phe-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Phe-Pro-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | Phe-Pro + 7-amino-4-trifluoromethylcoumarin | - |
? | |
3.4.21.B28 | Pro-Pro-7-amido-4-methylcoumarin + H2O | preferred substrate | Homo sapiens | Pro-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Ser-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ser-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Thr-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Thr-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Tyr-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Tyr-Pro + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B28 | Val-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Val-Pro + 7-amino-4-methylcoumarin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.B28 | homodimer | 2 * 100000, multiangle light scattering in combination with gel filtration chromatography and interferometric refractometry | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.19.1 | acylpeptide hydrolase | - |
synthetic construct |
3.4.19.1 | APH | - |
synthetic construct |
3.4.21.B28 | FAP | - |
Homo sapiens |
3.4.21.B28 | fibroblast activation protein protease | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B28 | 0.01 | - |
Lys-Ala-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 1.1 | - |
Phe-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 5.6 | - |
Gly-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 6.9 | - |
Ile-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens | |
3.4.21.B28 | 14.2 | - |
Ala-Pro-7-amido-4-trifluoromethylcoumarin | at 23°C in 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, pH 7.4 | Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.19.1 | 0.000575 | - |
Ac-Gly-prolineboronic acid | - |
synthetic construct | |
3.4.21.B28 | 0.000023 | - |
acetyl-Gly-Pro-boronic acid | - |
Homo sapiens |