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Literature summary extracted from

  • Pilquil, C.; Dewald, J.; Cherney, A.; Gorshkova, I.; Tigyi, G.; English, D.; Natarajan, V.; Brindley, D.N.
    Lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation (2006), J. Biol. Chem., 281, 38418-38429.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.4 additional information increasing the catalytic activity of LPP1 decreases the pertussis toxin-sensitive stimulation of fibroblast migration by lyso-phosphatidic acid and an lyso-phosphatidic acid-receptor agonist, that can not be dephosphorylated Rattus norvegicus

Application

EC Number Application Comment Organism
3.1.3.4 medicine LPP1 overexpressing fibroblasts show a severe inhibition of lyso-phosphatidic acid-induced migration in a wound healing assay. Intracellular actions of LPP1 play important functions in regulating lyso-phosphatidic acid-induced fibroblast migration through phospholipase D2, also controls platelet-derived growth factor-betabeta-induced phosphatidate formation Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.4 gene Lpp1, stable overexpression of wild-type and mutant LPP1 enzymes in Rat2 fibroblasts using a retroviral vector and puromycin selection Mus musculus
3.1.3.4 rat2 fibroblasts infected with adenoviral vectors containing LPP1-myc or LPP1(R217K)-myc. Rat2 fibroblasts stably overexpressing LPP1 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.4 additional information LPP1 overexpressing fibroblasts show a severe inhibition of LPA-induced migration not mediated through the degradation of extracellular LPA in a woundhealing assay, overview Mus musculus
3.1.3.4 R217K site-directed mutagenesis, inactive mutant Mus musculus
3.1.3.4 R217K inactive, mutant shows no decrease of lyso-phosphatidic acid-induced fibroblast migration Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.4 siRNA decrease of LPP1 mRNA by ca. 90%, knockdown of endogenous LPP1 activity by about 50% and increase of lyso-phosphatidic acid-induced migration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.4 phosphatidic acid + H2O Mus musculus lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview 1,2-diacylglycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.4 Mus musculus
-
-
-
3.1.3.4 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.4 1,2-dioleoyl-sn-glycerol-3 phosphate
-
 Mus musculus 1,2-dioleoyl-sn-glycerol + phosphate
-
 ?
3.1.3.4 lyso-phosphatidic acid + H2O
-
 Rattus norvegicus monoacylglycerol + phosphate
-
 ?
3.1.3.4 phosphatidic acid + H2O lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview Mus musculus 1,2-diacylglycerol + phosphate
-
 ?
3.1.3.4 phosphatidic acid + H2O
-
 Rattus norvegicus diacylglycerol + phosphate
-
 ?
3.1.3.4 sphingosine 1-phosphate + H2O
-
 Rattus norvegicus sphingosine + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.4 lipid phosphate phosphatase-1
-
 Mus musculus
3.1.3.4 lipid phosphate phosphatase-1
-
 Rattus norvegicus
3.1.3.4 LPP1
-
 Mus musculus
3.1.3.4 LPP1
-
 Rattus norvegicus