BRENDA - Enzyme Database

Lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation

Pilquil, C.; Dewald, J.; Cherney, A.; Gorshkova, I.; Tigyi, G.; English, D.; Natarajan, V.; Brindley, D.N.; J. Biol. Chem. 281, 38418-38429 (2006)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
increasing the catalytic activity of LPP1 decreases the pertussis toxin-sensitive stimulation of fibroblast migration by lyso-phosphatidic acid and an lyso-phosphatidic acid-receptor agonist, that can not be dephosphorylated
Rattus norvegicus
Application
EC Number
Application
Commentary
Organism
3.1.3.4
medicine
LPP1 overexpressing fibroblasts show a severe inhibition of lyso-phosphatidic acid-induced migration in a wound healing assay. Intracellular actions of LPP1 play important functions in regulating lyso-phosphatidic acid-induced fibroblast migration through phospholipase D2, also controls platelet-derived growth factor-betabeta-induced phosphatidate formation
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
gene Lpp1, stable overexpression of wild-type and mutant LPP1 enzymes in Rat2 fibroblasts using a retroviral vector and puromycin selection
Mus musculus
3.1.3.4
rat2 fibroblasts infected with adenoviral vectors containing LPP1-myc or LPP1(R217K)-myc. Rat2 fibroblasts stably overexpressing LPP1
Rattus norvegicus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
LPP1 overexpressing fibroblasts show a severe inhibition of LPA-induced migration not mediated through the degradation of extracellular LPA in a woundhealing assay, overview
Mus musculus
3.1.3.4
R217K
site-directed mutagenesis, inactive mutant
Mus musculus
3.1.3.4
R217K
inactive, mutant shows no decrease of lyso-phosphatidic acid-induced fibroblast migration
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
siRNA
decrease of LPP1 mRNA by ca. 90%, knockdown of endogenous LPP1 activity by about 50% and increase of lyso-phosphatidic acid-induced migration
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
phosphatidic acid + H2O
Mus musculus
lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview
1,2-diacylglycerol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Mus musculus
-
-
-
3.1.3.4
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lyso-phosphatidic acid + H2O
-
680723
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview
680723
Mus musculus
1,2-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680723
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680723
Mus musculus
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680723
Rattus norvegicus
sphingosine + phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
increasing the catalytic activity of LPP1 decreases the pertussis toxin-sensitive stimulation of fibroblast migration by lyso-phosphatidic acid and an lyso-phosphatidic acid-receptor agonist, that can not be dephosphorylated
Rattus norvegicus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.4
medicine
LPP1 overexpressing fibroblasts show a severe inhibition of lyso-phosphatidic acid-induced migration in a wound healing assay. Intracellular actions of LPP1 play important functions in regulating lyso-phosphatidic acid-induced fibroblast migration through phospholipase D2, also controls platelet-derived growth factor-betabeta-induced phosphatidate formation
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
gene Lpp1, stable overexpression of wild-type and mutant LPP1 enzymes in Rat2 fibroblasts using a retroviral vector and puromycin selection
Mus musculus
3.1.3.4
rat2 fibroblasts infected with adenoviral vectors containing LPP1-myc or LPP1(R217K)-myc. Rat2 fibroblasts stably overexpressing LPP1
Rattus norvegicus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
LPP1 overexpressing fibroblasts show a severe inhibition of LPA-induced migration not mediated through the degradation of extracellular LPA in a woundhealing assay, overview
Mus musculus
3.1.3.4
R217K
site-directed mutagenesis, inactive mutant
Mus musculus
3.1.3.4
R217K
inactive, mutant shows no decrease of lyso-phosphatidic acid-induced fibroblast migration
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
siRNA
decrease of LPP1 mRNA by ca. 90%, knockdown of endogenous LPP1 activity by about 50% and increase of lyso-phosphatidic acid-induced migration
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
phosphatidic acid + H2O
Mus musculus
lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview
1,2-diacylglycerol + phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lyso-phosphatidic acid + H2O
-
680723
Rattus norvegicus
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
lipid phosphate phosphatase-1 regulates lysophosphatidate-induced fibroblast migration by controlling phospholipase D2-dependent phosphatidate generation, LPP1 expression decreases PLD activity and PA accumulation after stimulating fibroblasts with either LPA or PDGF, but PLD-dependent PA formation Is only required for LPA-induced fibroblast migration, overview
680723
Mus musculus
1,2-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680723
Rattus norvegicus
diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
680723
Mus musculus
1,2-dioleoyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
sphingosine 1-phosphate + H2O
-
680723
Rattus norvegicus
sphingosine + phosphate
-
-
-
?