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Literature summary extracted from

  • Rangarajan, E.S.; Proteau, A.; Wagner, J.; Hung, M.N.; Matte, A.; Cygler, M.
    Structural snapshots of Escherichia coli histidinol phosphate phosphatase along the reaction pathway (2006), J. Biol. Chem., 281, 37930-37941.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.15 expressed in Escherichia coli strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.15 25% (w/v) polyethylene glycol 3350, 0.2M MgCl2 and 0.1 M Tris-HCl (pH 8.5) by the sitting drop vapor diffusion, or 30% (w/v) polyethylene glycol monomethyl ether, 0.05 M CaCl2, and 0.1 M Bis-Tris (pH 6.5) by the micro-batch method Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.15 D12A shows only traces of the wild type activity Escherichia coli
3.1.3.15 E18A shows about 5% of the wild-type activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.15 Ca2+ 0.1 mM, no activity Escherichia coli
3.1.3.15 EDTA 0.1 mM, no activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.15 0.041
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Zn2+ Escherichia coli
3.1.3.15 0.052
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Mn2+ Escherichia coli
3.1.3.15 0.054
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Co2+ Escherichia coli
3.1.3.15 0.054
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Mg2+ Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.15 Co2+ activating Escherichia coli
3.1.3.15 Mn2+ activating Escherichia coli
3.1.3.15 Zn2+ activating Escherichia coli
3.1.3.15 Zn2+ the presence of a structural Zn2+ ion stabilizes the conformation of an extended loop Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.15 19903
-
 2 * 19903, in the presence of Mg2+ and histidinol phosphate, ESI mass spectrometry Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.15 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.15 nickel-nitrilotriacetic acid column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.15 L-histidinol phosphate + H2O
-
 Escherichia coli L-histidinol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.15 dimer 2 * 19903, in the presence of Mg2+ and histidinol phosphate, ESI mass spectrometry Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.3.15 HisB-N N-terminal domain of the bifunctional enzyme HisB with histidinol phosphate phosphatase activity Escherichia coli
3.1.3.15 histidinol phosphate phosphatase
-
 Escherichia coli
3.1.3.15 HPase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.15 20 50 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Co2+ Escherichia coli
3.1.3.15 960
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Mn2+ Escherichia coli
3.1.3.15 1410
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Zn2+ Escherichia coli
3.1.3.15 2140
-
 L-histidinol phosphate in 25 mM Tris-HCl (pH 7.5), 70 nM enzyme, 0.025 mM Mg2+, and 0.2 mM histidinol phosphate, at 25°C, in the presence of 0.05 mM Mg2+ Escherichia coli