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Literature summary extracted from

  • Dougherty, M.J.; Boyd, J.M.; Downs, D.M.
    Inhibition of fructose-1,6-bisphosphatase by aminoimidazole carboxamide ribotide prevents growth of Salmonella enterica purH mutants on glycerol (2006), J. Biol. Chem., 281, 33892-33899.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 G20D kcat for fructose 1,6-bisphosphate is 1.62fold lower than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.86fold higherr than wild-type enzyme Salmonella enterica
3.1.3.11 T23I kcat for fructose 1,6-bisphosphate is 1.2fold lower than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.3fold lower than wild-type enzyme Salmonella enterica
3.1.3.11 T91I kcat for fructose 1,6-bisphosphate is 1.1fold higher than wild-type enzyme, Km-value for fructose 1,6-diphosphate is 1.14fold lower than wild-type enzyme Salmonella enterica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.11 aminoimidazole carboxamide ribotide inhibition of fructose-1,6-bisphosphatase by aminoimidazole carboxamide ribotide prevents growth of Salmonella enterica purH mutants on glycerol Salmonella enterica
3.1.3.11 AMP
-
 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 0.0085
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme T23I Salmonella enterica
3.1.3.11 0.0095
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme T19I Salmonella enterica
3.1.3.11 0.0108
-
 D-fructose 1,6-bisphosphate pH 7.5, wild-type enzyme Salmonella enterica
3.1.3.11 0.0201
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme G20D Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Salmonella enterica D-fructose 6-phosphate + phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.11 15
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme G20D Salmonella enterica
3.1.3.11 20.4
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme T23I Salmonella enterica
3.1.3.11 24.3
-
 D-fructose 1,6-bisphosphate pH 7.5, wild-type enzyme Salmonella enterica
3.1.3.11 26.8
-
 D-fructose 1,6-bisphosphate pH 7.5, mutant enzyme T19I Salmonella enterica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.11 0.0013
-
 AMP wild-type enzyme, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 0.00414
-
 AMP wild-type enzym, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 0.0373
-
 aminoimidazole carboxamide ribotide wild-type enzyme, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 0.155
-
 AMP mutant enzyme T19I, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 0.165
-
 aminoimidazole carboxamide ribotide wild-type enzym, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 0.404
-
 AMP mutant enzyme T19I, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 0.657
-
 AMP mutant enzyme G20S, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 1.16
-
 aminoimidazole carboxamide ribotide mutant enzyme T23I, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 1.35
-
 AMP mutant enzyme G20S, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 1.45
-
 AMP mutant enzyme T23I, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 1.7
-
 aminoimidazole carboxamide ribotide mutant enzyme T19I, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 2.16
-
 aminoimidazole carboxamide ribotide mutant enzyme G20S, competitive dissociation constant Kic Salmonella enterica
3.1.3.11 3.8
-
 aminoimidazole carboxamide ribotide mutant enzyme T23I, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 4.61
-
 aminoimidazole carboxamide ribotide mutant enzyme G20S, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 6.12
-
 AMP mutant enzyme T23I, uncompetitive dissociation constant Kiu Salmonella enterica
3.1.3.11 6.32
-
 aminoimidazole carboxamide ribotide mutant enzyme T19I, uncompetitive dissociation constant Kiu Salmonella enterica