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Literature summary extracted from

  • Angelo, P.F.; Lima, A.R.; Alves, F.M.; Blaber, S.I.; Scarisbrick, I.A.; Blaber, M.; Juliano, L.; Juliano, M.A.
    Substrate specificity of human kallikrein 6: salt and glycosaminoglycan activation effects (2006), J. Biol. Chem., 281, 3116-3126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.B10 chondroitin sulfate activates hK6 in a concentration-dependent manner Homo sapiens
3.4.21.B10 dermatan sulfate activates hK6 in a concentration-dependent manner Homo sapiens
3.4.21.B10 heparan sulfate activates hK6 in a concentration-dependent manner Homo sapiens
3.4.21.B10 heparin activates hK6 in a concentration-dependent manner Homo sapiens
3.4.21.B10 Sodium citrate reveals greatest activity at pH 7.5 Homo sapiens
3.4.21.B10 Sodium sulfate reveals greatest activity at pH 7.5 Homo sapiens

Application

EC Number Application Comment Organism
3.4.21.B10 additional information hK6 is involved in normal myelin turnover/demyelination processes, but it is unlikely to self-activate. Possibly it modulates ionotropic glutamate receptors and activates PAR 2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B10 expressed from a baculovirus/insect cell line system Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B10 Ca2+ 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 Homo sapiens
3.4.21.B10 K+ inhibits hK6 at pH 7.5 or 9.0 Homo sapiens
3.4.21.B10 Mg2+ 0.005 mM completely inhibits hK6 at pH 7.5 or 9.0 Homo sapiens
3.4.21.B10 NH4+ inhibits hK6 at pH 7.5 or 9.0 Homo sapiens
3.4.21.B10 Sodium citrate inhibits the enzyme at pH 9.0 Homo sapiens
3.4.21.B10 Sodium sulfate inhibits the enzyme at pH 9.0 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B10 0.0003
-
 o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.0003
-
 o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.001
-
 o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.0011
-
 o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.0014
-
 o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.0016
-
 o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.0028
-
 o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 0.004
-
 o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 9, without salt Homo sapiens
3.4.21.B10 0.009
-
 o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 9, without salt Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B10 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B10
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B10 alpha1-Antichymotrypsin + H2O hydrolysis of the FRET peptide derived from alpha1-antichymotrypsin Homo sapiens ?
-
 ?
3.4.21.B10 additional information hydrolysis of the FRET peptide series o-aminobenzoic acid-XLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KXRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRXSKQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-KLRSXKQ-N-[2,4-dinitrophenyl]ethylenediamine and FRET peptide derived from hK6 maturation/inactivation cleavage site or PAR. o-aminobenzoic acid-AFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine, o-aminobenzoic acid-VLFEKKVYLQ-N-[2,4-dinitrophenyl]ethylenediamine and o-aminobenzoic acid-GTEASVVAPQ-N-[2,4-dinitrophenyl]ethylenediamine are not hydrolyzed Homo sapiens ?
-
 ?
3.4.21.B10 myelin basic protein + H2O hydrolysis of the FRET peptide derived from human myelin basic protein Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O best synthetic substrate Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-EELAFKFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B10 plasminogen + H2O hydrolysis of the FRET peptide derived from plasminogen Homo sapiens ?
-
 ?
3.4.21.B10 precursor of the Abeta amyloid peptid + H2O hydrolysis of the FRET peptide derived from human precursor of the Abeta amyloid peptide Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B10 hK6
-
 Homo sapiens
3.4.21.B10 kallikrein 6
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B10 0.4
-
 o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 9, without salt Homo sapiens
3.4.21.B10 2.3
-
 o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 9, without salt Homo sapiens
3.4.21.B10 2.7
-
 o-aminobenzoic acid-AFRVGQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 7.1
-
 o-aminobenzoic acid-EEHAFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 7.1
-
 o-aminobenzoic acid-AFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 9.1
-
 o-aminobenzoic acid-EHSAFRFAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 9.2
-
 o-aminobenzoic acid-AFRLAQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 11.6
-
 o-aminobenzoic acid-AFRFSQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens
3.4.21.B10 14.2
-
 o-aminobenzoic acid-KLRSSKQ-N-[2,4-dinitrophenyl]ethylenediamine at pH 7.5, in the presence 2 M sodium citrate Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.B10 7 9
-
 Homo sapiens