BRENDA - Enzyme Database

Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast

Decker, B.L.; Wickner, W.T.; J. Biol. Chem. 281, 14523-14528 (2006)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
influence of enolase on membrane fusion of vacuoles and protein trafficking analyzed; influence of enolase on membrane fusion of vacuoles and protein trafficking analyzed
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli, His-tagging and GST-tagging of recombinant enolase protein; expressed in Escherichia coli, His-tagging and GST-tagging of recombinant enolase protein
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
impaired catalytic activity of enolase retains in vitro stimulation of fusion of isolated vacuoles, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion; impaired catalytic activity of enolase retains in vitro stimulation of vacuole fusion, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
small portion of enolase bound to vacuoles, in vitro stimulation of vacuole fusion determined; small portion of enolase bound to vacuoles, in vitro stimulation of vacuole fusion determined
Saccharomyces cerevisiae
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
P00924
-
-
4.2.1.11
Saccharomyces cerevisiae
P00925
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
gel filtration of recombinant protein; gel filtration of recombinant protein
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
in vitro stimulation of vacuole fusion by recombinant enolase determined, no stimulation solely by addition of substrate or product of enolase, catalytic activity independent of role in vacuole fusion, enolase-deficient vacuoles lack in vitro stimulation, enolase deficiency prevents normal protein sorting to the vacuole; in vitro stimulation of vacuole fusion by recombinant enolase determined, no stimulation solely by addition of substrate or product of enolase, catalytic activity independent of role in vacuole fusion, enolase-deficient vacuoles lack in vitro stimulation, enolase deficiency prevents normal protein sorting to the vacuole
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
680645
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
separated role of enolase besides function in glycolysis, stimulation of vacuole fusion and involvement in protein trafficking to vacuoles determined
680645
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.11
molecular biology
influence of enolase on membrane fusion of vacuoles and protein trafficking analyzed
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli, His-tagging and GST-tagging of recombinant enolase protein
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
additional information
impaired catalytic activity of enolase retains in vitro stimulation of vacuole fusion, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion
Saccharomyces cerevisiae
4.2.1.11
additional information
impaired catalytic activity of enolase retains in vitro stimulation of fusion of isolated vacuoles, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytosol
small portion of enolase bound to vacuoles, in vitro stimulation of vacuole fusion determined
Saccharomyces cerevisiae
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
gel filtration of recombinant protein
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
additional information
-
in vitro stimulation of vacuole fusion by recombinant enolase determined, no stimulation solely by addition of substrate or product of enolase, catalytic activity independent of role in vacuole fusion, enolase-deficient vacuoles lack in vitro stimulation, enolase deficiency prevents normal protein sorting to the vacuole
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
680645
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
separated role of enolase besides function in glycolysis, stimulation of vacuole fusion and involvement in protein trafficking to vacuoles determined
680645
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r