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Literature summary extracted from

  • Decker, B.L.; Wickner, W.T.
    Enolase activates homotypic vacuole fusion and protein transport to the vacuole in yeast (2006), J. Biol. Chem., 281, 14523-14528.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.11 molecular biology influence of enolase on membrane fusion of vacuoles and protein trafficking analyzed Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli, His-tagging and GST-tagging of recombinant enolase protein Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 additional information impaired catalytic activity of enolase retains in vitro stimulation of fusion of isolated vacuoles, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion Saccharomyces cerevisiae
4.2.1.11 additional information impaired catalytic activity of enolase retains in vitro stimulation of vacuole fusion, partial inactivity of enolase diminishes vacuole fusion, enolase-deficient vacuoles lacks in vitro stimulation of vacuole fusion Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytosol small portion of enolase bound to vacuoles, in vitro stimulation of vacuole fusion determined Saccharomyces cerevisiae 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Saccharomyces cerevisiae P00924
-
-
4.2.1.11 Saccharomyces cerevisiae P00925
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 gel filtration of recombinant protein Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 in vitro stimulation of vacuole fusion by recombinant enolase determined, no stimulation solely by addition of substrate or product of enolase, catalytic activity independent of role in vacuole fusion, enolase-deficient vacuoles lack in vitro stimulation, enolase deficiency prevents normal protein sorting to the vacuole Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r
4.2.1.11 2-phospho-D-glycerate separated role of enolase besides function in glycolysis, stimulation of vacuole fusion and involvement in protein trafficking to vacuoles determined Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Saccharomyces cerevisiae