BRENDA - Enzyme Database

Vacuole membrane topography of the DPP1-encoded diacylglycerol pyrophosphate phosphatase catalytic site from Saccharomyces cerevisiae

Han, G.S.; Johnston, C.N.; Carman, G.M.; J. Biol. Chem. 279, 5338-5345 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
3.1.3.81
additional information
the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase
Saccharomyces cerevisiae
3.1.3.81
gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Zn2+
zinc depletion increases the enzyme activity in vivo, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
3.1.3.81
Zn2+
zinc depletion increases the enzyme activity in vivo, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
vacuolar membrane
associated, the enzyme contains six putative transmembrane domains, the catalytic site is oriented to the cytosolic face of the vacuole membrane
Saccharomyces cerevisiae
5774
-
3.1.3.81
vacuolar membrane
associated, the enzyme contains six putative transmembrane domains, the catalytic site is oriented to the cytosolic face of the vacuole membrane
Saccharomyces cerevisiae
5774
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.4
phosphatidate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
phosphatidate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Saccharomyces cerevisiae
-
gene DPP1
-
3.1.3.4
Saccharomyces cerevisiae W303-1A
-
gene DPP1
-
3.1.3.81
Saccharomyces cerevisiae
-
gene DPP1
-
3.1.3.81
Saccharomyces cerevisiae W303-1A
-
gene DPP1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
preparation of vacuoles
Saccharomyces cerevisiae
3.1.3.81
preparation of vacuoles
Saccharomyces cerevisiae
Reaction
EC Number
Reaction
Commentary
Organism
3.1.3.4
a 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate
catalytic site structure within the transverse plane of the vacuole membrane, overview
Saccharomyces cerevisiae
3.1.3.81
1,2-diacyl-sn-glycerol 3-diphosphate + H2O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate
the bifunctional enzyme also catalyzes the phosphohydrolysis of phosphatidic acid to diacylglycerol, but is distinct from the phosphatidate phosphatase, EC 3.1.3.4, being Mg2+-independent and insensitive to N-ethylmaleimide, catalytic site structure within the transverse plane of the vacuole membrane, overview
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
1.4
-
recombinant vacuole membranes of intact vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.4
7
-
recombinant vacuole membranes of Triton X-100-ruptured vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.81
1.4
-
recombinant vacuole membranes of intact vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.81
7
-
recombinant vacuole membranes of Triton X-100-ruptured vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae W303-1A
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
More
the enzyme has six putative transmembrane domains and a hydrophilic region that contains a phosphatase motif required for its catalytic activity, membrane topology, overview
Saccharomyces cerevisiae
3.1.3.81
More
the enzyme has six putative transmembrane domains and a hydrophilic region that contains a phosphatase motif required for its catalytic activity, membrane topology, overview
Saccharomyces cerevisiae
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
assay at
Saccharomyces cerevisiae
3.1.3.81
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
5
-
assay at
Saccharomyces cerevisiae
3.1.3.81
5
-
assay at
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
3.1.3.81
additional information
the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase
Saccharomyces cerevisiae
3.1.3.81
gene DPP1, functional overexpression of HA-tagged enzyme in the DPP1-deficient mutant Saccharomyces cerevisiae strain DTY1 in vacuole membranes, the transcription factor Zap1p binds the DPP1 promoter and induces the expression of DGPP phosphatase
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Zn2+
zinc depletion increases the enzyme activity in vivo, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
3.1.3.81
Zn2+
zinc depletion increases the enzyme activity in vivo, stress condition of zinc depletion induces DPP1 expression
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
vacuolar membrane
associated, the enzyme contains six putative transmembrane domains, the catalytic site is oriented to the cytosolic face of the vacuole membrane
Saccharomyces cerevisiae
5774
-
3.1.3.81
vacuolar membrane
associated, the enzyme contains six putative transmembrane domains, the catalytic site is oriented to the cytosolic face of the vacuole membrane
Saccharomyces cerevisiae
5774
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.4
phosphatidate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
phosphatidate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae W303-1A
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
phosphatidate + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
preparation of vacuoles
Saccharomyces cerevisiae
3.1.3.81
preparation of vacuoles
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
1.4
-
recombinant vacuole membranes of intact vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.4
7
-
recombinant vacuole membranes of Triton X-100-ruptured vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.81
1.4
-
recombinant vacuole membranes of intact vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
3.1.3.81
7
-
recombinant vacuole membranes of Triton X-100-ruptured vacuoles from overexpressing transgenic mutant DTY1
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
3.1.3.4
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, reaction of EC 3.1.3.B2, and it then removes the phosphate from phosphatidate to form diacylglycerol
680628
Saccharomyces cerevisiae W303-1A
diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4
680628
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, which is the reaction of EC 3.1.3.4, zinc-mediated regulation, overview
680628
Saccharomyces cerevisiae W303-1A
phosphatidate + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
More
the enzyme has six putative transmembrane domains and a hydrophilic region that contains a phosphatase motif required for its catalytic activity, membrane topology, overview
Saccharomyces cerevisiae
3.1.3.81
More
the enzyme has six putative transmembrane domains and a hydrophilic region that contains a phosphatase motif required for its catalytic activity, membrane topology, overview
Saccharomyces cerevisiae
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
assay at
Saccharomyces cerevisiae
3.1.3.81
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
5
-
assay at
Saccharomyces cerevisiae
3.1.3.81
5
-
assay at
Saccharomyces cerevisiae