BRENDA - Enzyme Database

Mammalian Mg2+-independent phosphatidate phosphatase (PAP2) displays diacylglycerol pyrophosphate phosphatase activity

Dillon, D.A.; Chen, X.; Zeimetz, G.M.; Wu, W.I.; Waggoner, D.W.; Dewald, J.; Brindley, D.N.; Carman, G.M.; J. Biol. Chem. 272, 10361-10366 (1997)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.81
phosphatidate
slight activation of the DGPP phosphatases activity
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.81
ceramide 1-phosphate
-
Saccharomyces cerevisiae
3.1.3.81
lysophosphatidate
-
Saccharomyces cerevisiae
3.1.3.81
Mn2+
the inhibition of DGPP phosphatase activity by Mn2+ ions follows positive cooperative kinetics
Rattus norvegicus
3.1.3.81
additional information
no inhibition by N-ethylmaleimide and bromoenol lactone, and by EDTA and EGTA
Rattus norvegicus
3.1.3.81
phosphatidate
competitive versus diacylglycerol diphosphate
Rattus norvegicus
3.1.3.81
sphingosine 1-phosphate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.81
additional information
-
additional information
the enzyme shows high affinity for diacylglycerol diphosphate, kinetics, overview
Rattus norvegicus
3.1.3.81
additional information
-
additional information
kinetics
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.81
plasma membrane
-
Rattus norvegicus
5886
-
3.1.3.81
plasma membrane
-
Saccharomyces cerevisiae
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.81
additional information
no Mg2+ or other divalent cations required for diacylglycerol diphosphate hydrolase activity
Rattus norvegicus
3.1.3.81
additional information
no Mg2+ or other divalent cations required
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.81
diacylglycerol diphosphate + H2O
Rattus norvegicus
preferred substrate
phosphatidate + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
preferred substrate
phosphatidate + phosphate
-
-
?
3.1.3.81
additional information
Rattus norvegicus
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
?
-
-
-
3.1.3.81
additional information
Saccharomyces cerevisiae
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.81
Rattus norvegicus
-
-
-
3.1.3.81
Saccharomyces cerevisiae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.81
liver
-
Rattus norvegicus
-
3.1.3.81
liver
-
Saccharomyces cerevisiae
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate
680603
Rattus norvegicus
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate
680603
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate, the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4
680603
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate, the bifunctional PAP2 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4
680603
Rattus norvegicus
phosphatidate + phosphate
-
-
-
?
3.1.3.81
additional information
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
680603
Rattus norvegicus
?
-
-
-
-
3.1.3.81
additional information
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
680603
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.81
30
-
assay at
Rattus norvegicus
3.1.3.81
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.81
5
6
DGPP phosphatase activity
Rattus norvegicus
3.1.3.81
5.5
6.5
assay at
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.81
additional information
-
additional information
the inhibition of DGPP phosphatase activity by Mn2+ ions follows positive cooperative kinetics
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.81
phosphatidate
slight activation of the DGPP phosphatases activity
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.81
ceramide 1-phosphate
-
Saccharomyces cerevisiae
3.1.3.81
lysophosphatidate
-
Saccharomyces cerevisiae
3.1.3.81
Mn2+
the inhibition of DGPP phosphatase activity by Mn2+ ions follows positive cooperative kinetics
Rattus norvegicus
3.1.3.81
additional information
no inhibition by N-ethylmaleimide and bromoenol lactone, and by EDTA and EGTA
Rattus norvegicus
3.1.3.81
phosphatidate
competitive versus diacylglycerol diphosphate
Rattus norvegicus
3.1.3.81
sphingosine 1-phosphate
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.81
additional information
-
additional information
the inhibition of DGPP phosphatase activity by Mn2+ ions follows positive cooperative kinetics
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.81
additional information
-
additional information
the enzyme shows high affinity for diacylglycerol diphosphate, kinetics, overview
Rattus norvegicus
3.1.3.81
additional information
-
additional information
kinetics
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.81
plasma membrane
-
Rattus norvegicus
5886
-
3.1.3.81
plasma membrane
-
Saccharomyces cerevisiae
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.81
additional information
no Mg2+ or other divalent cations required for diacylglycerol diphosphate hydrolase activity
Rattus norvegicus
3.1.3.81
additional information
no Mg2+ or other divalent cations required
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.81
diacylglycerol diphosphate + H2O
Rattus norvegicus
preferred substrate
phosphatidate + phosphate
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
Saccharomyces cerevisiae
preferred substrate
phosphatidate + phosphate
-
-
?
3.1.3.81
additional information
Rattus norvegicus
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
?
-
-
-
3.1.3.81
additional information
Saccharomyces cerevisiae
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.81
liver
-
Rattus norvegicus
-
3.1.3.81
liver
-
Saccharomyces cerevisiae
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate
680603
Rattus norvegicus
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate
680603
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate, the bifunctional DPP1 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4
680603
Saccharomyces cerevisiae
phosphatidate + phosphate
-
-
-
?
3.1.3.81
diacylglycerol diphosphate + H2O
preferred substrate, the bifunctional PAP2 catalyzes the removal of the beta-phosphate from diacylglycerol diphosphate to form phosphatidate, and it then removes the phosphate from phosphatidate to form diacylglycerol, reaction of EC 3.1.3.4
680603
Rattus norvegicus
phosphatidate + phosphate
-
-
-
?
3.1.3.81
additional information
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
680603
Rattus norvegicus
?
-
-
-
-
3.1.3.81
additional information
the enzyme is involved in regulation of the cellular levels of diacylglycerol diphosphate, phosphatidate, and diacylglycerol
680603
Saccharomyces cerevisiae
?
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.3.81
30
-
assay at
Rattus norvegicus
3.1.3.81
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.81
5
6
DGPP phosphatase activity
Rattus norvegicus
3.1.3.81
5.5
6.5
assay at
Saccharomyces cerevisiae