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Literature summary extracted from

  • Ahmad, B.; Shamim, T.A.; Haq, S.K.; Khan, R.H.
    Identification and characterization of functional intermediates of stem bromelain during urea and guanidine hydrochloride unfolding (2007), J. Biochem., 141, 251-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.32 Guanidine-HCl at 2 mM Ananas comosus
3.4.22.32 Urea at 5 M and 8-9 M Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 Guanidine-HCl complete loss of activity at 6 M guanidine-HCl Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.129
-
 casein native enzyme, at pH 7.0 Ananas comosus
3.4.22.32 0.217
-
 casein native enzyme, in the presence of 5 M urea at pH 7.0 Ananas comosus
3.4.22.32 0.221
-
 casein native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 Ananas comosus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.32 Ethanol enzyme stabilized in 60-70% ethanol is non-functional, but retains all the elements of secondary structure Ananas comosus
3.4.22.32 guanidine-HCl activity-enhanced state in the presence of 2 M guanidine-HCl Ananas comosus
3.4.22.32 urea activity-enhanced state in the presence of 5 M and 8-9 M urea Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.32 Bromelain
-
 Ananas comosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 0.41
-
 casein native enzyme, at pH 7.0 Ananas comosus
3.4.22.32 0.97
-
 casein native enzyme, in the presence of 5 M urea at pH 7.0 Ananas comosus
3.4.22.32 0.99
-
 casein native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 Ananas comosus
3.4.22.32 11.72
-
 casein native enzyme, at pH 7.0 Ananas comosus
3.4.22.32 17.33
-
 casein native enzyme, in the presence of 5 M urea at pH 7.0 Ananas comosus
3.4.22.32 18.08
-
 casein native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 Ananas comosus