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Literature summary extracted from

  • Kawamura, S.; Ohno, K.; Ohkuma, M.; Chijiiwa, Y.; Torikata, T.
    Experimental verification of the crucial roles of Glu73 in the catalytic activity and structural stability of goose type lysozyme (2006), J. Biochem., 140, 75-85.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.17 expression in Pichia pastoris Struthio camelus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.17 E73A no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 3.2°C as compared to wild-type value Struthio camelus
3.2.1.17 E73D detectable level of activity, but lytic activity against Micrococcus lysodeikticus is drastically reduced as compared to wild-type activity. Decrease in Tm-value is 6.1°C as compared to wild-type value Struthio camelus
3.2.1.17 E73Q no hydrolysis of N,N',N'',N''',N''''-pentaacetylchitopentaose at 50°C for 48 h. Decrease in Tm-value is 4.0°C as compared to wild-type value Struthio camelus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Struthio camelus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.17
-
 Struthio camelus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.17 when the thermal denaturation curves are analyzed in the absence of guanidine hydrochloride, the reversibility calculated is 76% for the wild-type enzyme, 80% for E73D, 80% for E73Q and 79% for E73A. In the presence of 0.5 M guanidine hydrochloride, the reversibility of the thermal unfolding for the wild type and mutant proteins is more than 95% Struthio camelus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.17 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O wild-type protein hydrolyzes N,N',N'',N''',N''''-pentaacetylchitopentaose almost completely on 140 min reaction. N,N',N'',N'''-tetraacetylchitotetraose is is hydrolyzed mainly to N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose with much less cleavage into GlcNAc + N,N',N'',N'''-tetraacetylchitotetraose Struthio camelus N,N'-diacetylchitobiose + p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose
-
 ?
3.2.1.17 peptidoglycan + H2O lytic activity against Micrococcus lysodeikticus Struthio camelus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.17 goose type lysozyme
-
 Struthio camelus
3.2.1.17 OEL
-
 Struthio camelus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.17 55
-
 Tm-value for mutant enzyme E73S is 54.5°C Struthio camelus
3.2.1.17 57
-
 Tm-value for mutant E73A is 57.4°C. Tm-value for mutant E73Q is 56.6°C Struthio camelus
3.2.1.17 61
-
 Tm-value for wild-type enzyme is 60.6°C Struthio camelus