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Literature summary extracted from

  • Mashhadi, Z.; Zhang, H.; Xu, H.; White, R.H.
    Identification and characterization of an archaeon-specific riboflavin kinase (2008), J. Bacteriol., 190, 2615-2618.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.161 expression in Escherichia coli Methanocaldococcus jannaschii
2.7.1.161 recombinant expression of the MJ0056 gene in Escherichia coli Methanocaldococcus jannaschii
2.7.1.161 recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide in the Escherichia coli cell extract Methanocaldococcus jannaschii

General Stability

EC Number General Stability Organism
2.7.1.161 10 min at room temperature, followed by dilution in acetate buffer (pH 6.0) Methanocaldococcus jannaschii
2.7.1.161 the enzyme retains 40% (0.13 nmol/min) of its activity compared to the control (0.32 nmol/min) after treatment with 0.1 M HCl (pH 1.05) for Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.161 Co2+ 1.6 mM, 40% inhibition; 1.6 mM, 61% inhibition Methanocaldococcus jannaschii
2.7.1.161 Cu2+ 1.6 mM, 3% inhibition; 1.6 mM, 97% inhibition Methanocaldococcus jannaschii
2.7.1.161 Mg2+ 1.6 mM, 15% inhibition Methanocaldococcus jannaschii
2.7.1.161 Mn2+ 1.6 mM, 50% inhibition Methanocaldococcus jannaschii
2.7.1.161 Ni2+ 1.6 mM, 43% inhibition; 1.6 mM, 60% inhibition Methanocaldococcus jannaschii
2.7.1.161 Zn2+ 1.6 mM, 17% inhibition; 1.6 mM, 83% inhibition Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.161 0.159
-
 riboflavin
-
 Methanocaldococcus jannaschii
2.7.1.161 0.159
-
 riboflavin pH 7.2, 70°C Methanocaldococcus jannaschii
2.7.1.161 1.8
-
 CTP
-
 Methanocaldococcus jannaschii
2.7.1.161 1.8
-
 CTP pH 7.2, 70°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.161 Mg2+ the enzyme contains 4 to 5 mol of Mg Methanocaldococcus jannaschii
2.7.1.161 Mg2+ contains 4 to 5 mol of Mg per mol of protein Methanocaldococcus jannaschii
2.7.1.161 Mg2+ contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein Methanocaldococcus jannaschii
2.7.1.161 additional information absence of a requirement for added metal ion to catalyze the formation of FMN Methanocaldococcus jannaschii
2.7.1.161 additional information purified recombinant enzyme shows no requirement for added metal ion to catalyze the formation of FMN Methanocaldococcus jannaschii
2.7.1.161 Zn2+ contains 0.0013 mol of Zn per mol of protein Methanocaldococcus jannaschii
2.7.1.161 Zn2+ contains 4-5 mol of Mg2+ and 0.0013 mol of Zn2+ per mol of protein Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.161 15000
-
 1 * 15000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.161 15218
-
 1 * 15218, electrospray mass spectrometry Methanocaldococcus jannaschii
2.7.1.161 19700
-
 gel filtration Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.161 Methanocaldococcus jannaschii Q60365
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.161 recombinant Methanocaldococcus jannaschii
2.7.1.161 recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.161 ATP + riboflavin 30% of the activity with CTP Methanocaldococcus jannaschii ADP + FMN
-
 ?
2.7.1.161 ATP + riboflavin 30% of the activity with ATP Methanocaldococcus jannaschii ADP + riboflavin 5'-phosphate
-
 ?
2.7.1.161 CTP + riboflavin most active with CTP as the substrate Methanocaldococcus jannaschii CDP + riboflavin 5'-phosphate
-
 ?
2.7.1.161 CTP + riboflavin
-
 Methanocaldococcus jannaschii CDP + FMN
-
 ?
2.7.1.161 GTP + riboflavin 11% of the activity with ATP Methanocaldococcus jannaschii GDP + riboflavin 5'-phosphate
-
 ?
2.7.1.161 GTP + riboflavin 11% of the activity with CTP Methanocaldococcus jannaschii GDP + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.161 monomer 1 * 15000, SDS-PAGE Methanocaldococcus jannaschii
2.7.1.161 monomer 1 * 15218, electrospray mass spectrometry Methanocaldococcus jannaschii
2.7.1.161 monomer 1 * 19700 Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.1.161 MJ0056
-
 Methanocaldococcus jannaschii
2.7.1.161 RibK
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.161 100
-
 10 min, stable Methanocaldococcus jannaschii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.161 1
-
 10 min at room temperature, 60% loss of activity Methanocaldococcus jannaschii