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Literature summary extracted from

  • Ohshima, N.; Yamashita, S.; Takahashi, N.; Kuroishi, C.; Shiro, Y.; Takio, K.
    Escherichia coli cytosolic glycerophosphodiester phosphodiesterase (UgpQ) requires Mg2+, Co2+, or Mn2+ for its enzyme activity (2008), J. Bacteriol., 190, 1219-1223.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.46 expressed in Escherichia coli BL21(DE3) Escherichia coli K-12
3.1.4.46 protein in Escherichia coli BL21(DE3) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.46 0.22
-
 glycerophosphoethanolamine
-
 Escherichia coli K-12
3.1.4.46 0.22
-
 glycerophosphoethanolamine 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 0.39
-
 glycerophosphoinositol
-
 Escherichia coli K-12
3.1.4.46 0.39
-
 glycerophosphoinositol 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 0.62
-
 glycerophosphoglycerol
-
 Escherichia coli K-12
3.1.4.46 0.62
-
 glycerophosphoglycerol 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 0.66
-
 glycerophosphoserine
-
 Escherichia coli K-12
3.1.4.46 0.66
-
 glycerophosphoserine 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 2
-
 glycerophosphocholine
-
 Escherichia coli K-12
3.1.4.46 2
-
 glycerophosphocholine 5 mM Mg2+, pH 7.4, 35°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.46 cytosol
-
 Escherichia coli K-12 5829
-
3.1.4.46 cytosol
-
 Escherichia coli 5829
-
3.1.4.46 periplasm
-
 Escherichia coli
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.46 Ca2+ required Escherichia coli
3.1.4.46 Co2+ can replace Mg2+ Escherichia coli
3.1.4.46 Co2+ requires Mg2+, Mn2+, or Co2+ Escherichia coli K-12
3.1.4.46 Mg2+ required Escherichia coli
3.1.4.46 Mg2+ requires Mg2+, Mn2+, or Co2+ Escherichia coli K-12
3.1.4.46 Mn2+ can replace Mg2+ Escherichia coli
3.1.4.46 Mn2+ requires Mg2+, Mn2+, or Co2+ Escherichia coli K-12
3.1.4.46 additional information Ca2+, Cu2+, Ni2+, Cd2+, Zn2+, and Fe2+ are ineffective Escherichia coli
3.1.4.46 additional information Mg2+, Mn2+, Co2+, Cu2+, Ni2+, Cd2+, Zn2+, and Fe2+ are ineffective Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.46 27000
-
 x * 27000, SDS-PAGE Escherichia coli K-12
3.1.4.46 27000
-
 x * 27000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.46 glycerophosphocholine + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + choline
-
 ?
3.1.4.46 glycerophosphodiester + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + alcohol sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids ?
3.1.4.46 glycerophosphodiester + H2O Escherichia coli UgpQ accumulates under conditions of phosphate starvation suggesting a role in the utilization of phosphate from glycerophosphodiesters sn-glycerol 3-phosphate + alcohol sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids ?
3.1.4.46 glycerophosphoethanolamine + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + ethanolamine
-
 ?
3.1.4.46 glycerophosphoglycerol + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + glycerol
-
 ?
3.1.4.46 glycerophosphoinositol + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + inositol
-
 ?
3.1.4.46 glycerophosphoserine + H2O Escherichia coli
-
 sn-glycerol 3-phosphate + serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.46 Escherichia coli P09394
-
-
3.1.4.46 Escherichia coli P10908
-
-
3.1.4.46 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.46 ion exchange chromatography, hydroxyapatite chromatography, gel filtration Escherichia coli K-12
3.1.4.46 recombinant protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.46 glycerophosphocholine + H2O
-
 Escherichia coli K-12 sn-glycerol 3-phosphate + choline
-
 ?
3.1.4.46 glycerophosphocholine + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + choline
-
 ?
3.1.4.46 glycerophosphodiester + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + alcohol sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids ?
3.1.4.46 glycerophosphodiester + H2O UgpQ accumulates under conditions of phosphate starvation suggesting a role in the utilization of phosphate from glycerophosphodiesters Escherichia coli sn-glycerol 3-phosphate + alcohol sn-glycerol-3-phosphate supply is essential for the de novo synthesis of glycerophospholipids ?
3.1.4.46 glycerophosphoethanolamine + H2O
-
 Escherichia coli K-12 sn-glycerol 3-phosphate + ethanolamine
-
 ?
3.1.4.46 glycerophosphoethanolamine + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + ethanolamine
-
 ?
3.1.4.46 glycerophosphoglycerol + H2O
-
 Escherichia coli K-12 sn-glycerol 3-phosphate + glycerol
-
 ?
3.1.4.46 glycerophosphoglycerol + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + glycerol
-
 ?
3.1.4.46 glycerophosphoinositol + H2O
-
 Escherichia coli K-12 ?
-
 ?
3.1.4.46 glycerophosphoinositol + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + inositol
-
 ?
3.1.4.46 glycerophosphoserine + H2O
-
 Escherichia coli K-12 ?
-
 ?
3.1.4.46 glycerophosphoserine + H2O
-
 Escherichia coli sn-glycerol 3-phosphate + serine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.46 ? x * 27000, SDS-PAGE Escherichia coli K-12
3.1.4.46 ? x * 27000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.4.46 GlpQ periplasmic enzyme, requires Ca2+ Escherichia coli
3.1.4.46 glycerophosphodiester phosphodiesterase
-
 Escherichia coli K-12
3.1.4.46 UgpQ cytosolic enzyme, requires Mg2+, Mn2+, or Co2+ Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.46 1.1
-
 glycerophosphoethanolamine
-
 Escherichia coli K-12
3.1.4.46 1.1
-
 glycerophosphoethanolamine 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 1.8
-
 glycerophosphoinositol
-
 Escherichia coli K-12
3.1.4.46 1.8
-
 glycerophosphoinositol 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 2.8
-
 glycerophosphoserine
-
 Escherichia coli K-12
3.1.4.46 2.8
-
 glycerophosphoserine UgpQ, 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 3.2
-
 glycerophosphocholine
-
 Escherichia coli K-12
3.1.4.46 3.2
-
 glycerophosphocholine 5 mM Mg2+, pH 7.4, 35°C Escherichia coli
3.1.4.46 3.3
-
 glycerophosphoglycerol
-
 Escherichia coli K-12
3.1.4.46 3.3
-
 glycerophosphoglycerol 5 mM Mg2+, pH 7.4, 35°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.46 7.5
-
-
 Escherichia coli K-12
3.1.4.46 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.46 5.5 9.5
-
 Escherichia coli K-12
3.1.4.46 6 9
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.46 1.6
-
 glycerophosphocholine
-
 Escherichia coli K-12
3.1.4.46 4.2
-
 glycerophosphoserine
-
 Escherichia coli K-12
3.1.4.46 4.7
-
 glycerophosphoinositol
-
 Escherichia coli K-12
3.1.4.46 4.8
-
 glycerophosphoethanolamine
-
 Escherichia coli K-12
3.1.4.46 5.4
-
 glycerophosphoglycerol
-
 Escherichia coli K-12