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Literature summary extracted from

  • Labes, A.; Schoenheit, P.
    Unusual starch degradation pathway via cyclodextrins in the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2007), J. Bacteriol., 189, 8901-8913.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.54 biotechnology Archaeoglobus fulgidus utilizes an unusual pathway of starch degradation involving cyclodextrins as intermediates, extracellular cyclodextrins are transported into the cell and linearized via a CDase Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 EDTA at 20 mM Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.54 0.02
-
gamma-cyclodextrin at 80°C, pH 4.5 Archaeoglobus fulgidus
3.2.1.54 0.26
-
alpha-cyclodextrin at 80°C, pH 4.5 Archaeoglobus fulgidus
3.2.1.54 0.63
-
beta-cyclodextrin at 80°C, pH 4.5 Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Ca2+ divalent cations required Archaeoglobus fulgidus
3.2.1.54 Co2+ divalent cations required Archaeoglobus fulgidus
3.2.1.54 Cu2+ inhibitory at 1 mM Archaeoglobus fulgidus
3.2.1.54 Mg2+ divalent cations required Archaeoglobus fulgidus
3.2.1.54 Ni2+ inhibitory at 1 mM Archaeoglobus fulgidus
3.2.1.54 Zn2+ inhibitory at 1 mM Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 73000
-
SDS-PAGE Archaeoglobus fulgidus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Archaeoglobus fulgidus
-
-
-
3.2.1.54 Archaeoglobus fulgidus 7324
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 by Q-sepharose column, DEAE-Sepharose column, Bioprep SE 1000/17 and UNO S1 column chromatography Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 0.066
-
in the crude extract at 80°C Archaeoglobus fulgidus
3.2.1.54 36
-
after purification at 80°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclodextrin + H2O
-
Archaeoglobus fulgidus maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?
3.2.1.54 alpha-cyclodextrin + H2O
-
Archaeoglobus fulgidus 7324 maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?
3.2.1.54 beta-cyclodextrin + H2O
-
Archaeoglobus fulgidus maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?
3.2.1.54 beta-cyclodextrin + H2O
-
Archaeoglobus fulgidus 7324 maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?
3.2.1.54 cyclomaltodextrin + H2O no avtivity with activity with starch, pullulan, amylose, amylopectin, or oligosaccharides as substrates Archaeoglobus fulgidus linear maltodextrin
-
?
3.2.1.54 cyclomaltodextrin + H2O no avtivity with activity with starch, pullulan, amylose, amylopectin, or oligosaccharides as substrates Archaeoglobus fulgidus 7324 linear maltodextrin
-
?
3.2.1.54 gamma-cyclodextrin + H2O preferred substrate Archaeoglobus fulgidus maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?
3.2.1.54 gamma-cyclodextrin + H2O preferred substrate Archaeoglobus fulgidus 7324 maltooligosaccharides maltohexaose, maltoheptaose, maltooctaose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 monomer 1*73000 Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
Archaeoglobus fulgidus
3.2.1.54 cyclodextrinase
-
Archaeoglobus fulgidus
3.2.1.54 cyclomaltodextrinase
-
Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 90
-
up to 90° Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 4.5
-
-
Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 4 7
-
Archaeoglobus fulgidus