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Literature summary extracted from

  • Spaepen, S.; Versees, W.; Gocke, D.; Pohl, M.; Steyaert, J.; Vanderleyden, J.
    Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense (2007), J. Bacteriol., 189, 7626-7633.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.43 expressed in Escherichia coli BL21-CodonPlus(DE3)-RP cells Azospirillum brasilense

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.43 additional information an ipdC-knockout mutant produces only 10% (w/v) of the wild type indole-3-acetic acid production level Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.43 0.13
-
 Indole-3-pyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 0.3
-
 5-phenyl-2-oxopentanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 1.08
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 1.91
-
 4-phenyl-2-oxobutanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 5.51
-
 2-ketohexanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.43 60000
-
 4 * 60000, gel filtration Azospirillum brasilense
4.1.1.43 240600
-
 gel filtration, recombinant enzyme Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.43 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.43 HisTrap HP column chromatography and Superdex G200 gel filtration Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.43 2-ketohexanoic acid 1% activity compared to phenylpyruvate Azospirillum brasilense pentanal + CO2
-
 ?
4.1.1.43 4-phenyl-2-oxobutanoic acid 2.3% activity compared to phenylpyruvate Azospirillum brasilense 3-phenylpropanal + CO2
-
 ?
4.1.1.43 5-phenyl-2-oxopentanoic acid 110% activity compared to phenylpyruvate Azospirillum brasilense 4-phenylbutanal + CO2
-
 ?
4.1.1.43 indole-3-pyruvate 10.4% activity compared to phenylpyruvate Azospirillum brasilense indoleacetaldehyde + CO2
-
 ?
4.1.1.43 additional information no activity with benzoylformate and indole-glyoxylic acid Azospirillum brasilense ?
-
 ?
4.1.1.43 Phenylpyruvate 100% activity Azospirillum brasilense Phenylacetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.43 tetramer 4 * 60000, gel filtration Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
4.1.1.43 phenylpyruvate decarboxylase formerly classified as indole-3-pyruvate decarboxylase (ipdC) Azospirillum brasilense

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.43 additional information
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 0.07
-
 Indole-3-pyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 0.21
-
 4-phenyl-2-oxobutanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 0.29
-
 2-ketohexanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 1.7
-
 5-phenyl-2-oxopentanoic acid in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense
4.1.1.43 5.56
-
 phenylpyruvate in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.43 thiamine diphosphate 0.1 mM Azospirillum brasilense