Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, C.; Beattie, G.A.
    Characterization of the osmoprotectant transporter OpuC from Pseudomonas syringae and demonstration that cystathionine-beta-synthase domains are required for its osmoregulatory function (2007), J. Bacteriol., 189, 6901-6912.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.22 analysis usefulness of the CBS domains as predictors of osmoregulatory activity Pseudomonas syringae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.9 expressed in Escherichia coli, DH5-alpha used as host for cloning, MG1655 used as host for mutagenesis Pseudomonas syringae

Protein Variants

EC Number Protein Variants Comment Organism
7.6.2.9 additional information deletion mutants of OpuC generated, complementation of deletion mutants shown Pseudomonas syringae

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.9 additional information competition assays performed, compounds inhibiting the uptake of choline and glycine betaine summarized Pseudomonas syringae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.9 0.0037
-
 glycine betaine kinetics of OpuC-mediated uptake, in 50 mM of phosphate buffer containing 0.5 M of NaCl, 0.2% glucose, pH 7, low-affinity transport of choline determined Pseudomonas syringae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.22 additional information Pseudomonas syringae cystathionine-beta-synthase domains in the ATP-binding component of OpuC are required for transporter function ?
-
 ?
7.6.2.9 ATP + H2O Pseudomonas syringae
-
 ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Pseudomonas syringae
-
-
-
7.6.2.9 Pseudomonas syringae Q87WH5 ATP-binding protein, gene PSPTO4575, OpuCA; pv. tomato, strain DC3000
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.9 gel filtration, recombinant protein Pseudomonas syringae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6.2.9 additional information
-
 osmoprotection assays and transport assays applied, kinetic studies with radiolabeled substrates performed, OpuC identified as sole transporter for glycine betaine and as one of multiple transporters for choline under high osmolarity, close relation to OpuC transporters of Bacillus subtilis and Listeria monocytogenes determined, cystathionine-beta-synthase domain identification and homology search performed Pseudomonas syringae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 additional information cystathionine-beta-synthase domains in the ATP-binding component of OpuC are required for transporter function Pseudomonas syringae ?
-
 ?
7.6.2.9 ATP + H2O
-
 Pseudomonas syringae ADP + phosphate
-
 ?
7.6.2.9 ATP + H2O + glycine betaine/out enhanced osmoprotection found to be correlated with higher capacity for choline uptake than for glycine uptake, broad substrate specificity including acetylcholine, carnitine, and proline betaine determined, cystathionine-beta-synthase domains shown to be essential for osmoregulatory function Pseudomonas syringae ADP + phosphate + glycine betaine/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.22 osmoprotectant transporter OpuC
-
 Pseudomonas syringae
7.6.2.9 OpuC
-
 Pseudomonas syringae