BRENDA - Enzyme Database

Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcB

Ramirez-Trujillo, J.A.; Encarnacion, S.; Salazar, E.; de los Santos, A.G.; Dunn, M.F.; Emerich, D.W.; Calva, E.; Hernandez-Lucas, I.; J. Bacteriol. 189, 5875-5884 (2007)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.9
additional information
Sinorhizobium meliloti
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
?
-
-
-
2.3.3.9
additional information
Sinorhizobium meliloti Rm5000
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.3.9
Sinorhizobium meliloti
-
-
-
4.1.3.1
Sinorhizobium meliloti
-
-
-
4.1.3.1
Sinorhizobium meliloti Rm5000
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
0.138
-
in 50 mM MOPS buffer (pH 7.3), 5 mM MgCl2, 1 mM EDTA, 4 mM phenylhydrazine HCl
Sinorhizobium meliloti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.9
additional information
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
680497
Sinorhizobium meliloti
?
-
-
-
-
2.3.3.9
additional information
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
680497
Sinorhizobium meliloti Rm5000
?
-
-
-
-
4.1.3.1
isocitrate
-
680497
Sinorhizobium meliloti
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680497
Sinorhizobium meliloti Rm5000
succinate + glyoxylate
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.3.9
additional information
Sinorhizobium meliloti
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
?
-
-
-
2.3.3.9
additional information
Sinorhizobium meliloti Rm5000
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
0.138
-
in 50 mM MOPS buffer (pH 7.3), 5 mM MgCl2, 1 mM EDTA, 4 mM phenylhydrazine HCl
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.3.9
additional information
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
680497
Sinorhizobium meliloti
?
-
-
-
-
2.3.3.9
additional information
glcB (encoding malate synthase), in the absence of the 240-bp open reading frame SMc00767 is required for acetate metabolism. Malate synthase is not involved in nodulation or nitrogen fixation in the interaction of Sinorhizobium meliloti with alfalfa plants. glcB is dispensable in plant-microbe interactions
680497
Sinorhizobium meliloti Rm5000
?
-
-
-
-
4.1.3.1
isocitrate
-
680497
Sinorhizobium meliloti
succinate + glyoxylate
-
-
-
?
4.1.3.1
isocitrate
-
680497
Sinorhizobium meliloti Rm5000
succinate + glyoxylate
-
-
-
?