BRENDA - Enzyme Database

Iron-dependent RNA-binding activity of Mycobacterium tuberculosis aconitase

Banerjee, S.; Nandyala, A.K.; Raviprasad, P.; Ahmed, N.; Hasnain, S.E.; J. Bacteriol. 189, 4046-4052 (2007)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.56
-
isocitrate
pH 8.0, 25°C
Mycobacterium tuberculosis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
Mycobacterium tuberculosis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
100000
-
gel filtration
Mycobacterium tuberculosis
4.2.1.3
102000
-
1 * 102000, SDS-PAGE
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Mycobacterium tuberculosis
-
bifunctional enzyme with aconitase activity and working as iron-responsive protein. Since iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
118
-
pH 8.0, 25°C
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
680491
Mycobacterium tuberculosis
cis-aconitate + H2O
-
-
-
?
4.2.1.3
additional information
in addition to aconitase activity, enzyme binds with high specificity to iron-responsive element-like RNA sequences. Iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
680491
Mycobacterium tuberculosis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
1 * 102000, SDS-PAGE
Mycobacterium tuberculosis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
-
Mycobacterium tuberculosis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
7
10
-
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
0.56
-
isocitrate
pH 8.0, 25°C
Mycobacterium tuberculosis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
Mycobacterium tuberculosis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.3
100000
-
gel filtration
Mycobacterium tuberculosis
4.2.1.3
102000
-
1 * 102000, SDS-PAGE
Mycobacterium tuberculosis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.3
118
-
pH 8.0, 25°C
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
isocitrate
-
680491
Mycobacterium tuberculosis
cis-aconitate + H2O
-
-
-
?
4.2.1.3
additional information
in addition to aconitase activity, enzyme binds with high specificity to iron-responsive element-like RNA sequences. Iron is required for aconitase activity, but inhibits the RNA-binding activity, the two activities are mutually exclusive
680491
Mycobacterium tuberculosis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.3
monomer
1 * 102000, SDS-PAGE
Mycobacterium tuberculosis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
8
-
-
Mycobacterium tuberculosis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
7
10
-
Mycobacterium tuberculosis