EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.1 | gene catA, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree, identification of the cat gene cluster of strain MT1 | Pseudomonas reinekei |
1.13.11.1 | gene salD, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree | Pseudomonas reinekei |
1.14.13.1 | gene salA, DNA and amino acid sequence determination and analysis, phylogenetic analysis | Pseudomonas reinekei |
5.5.1.1 | part of gene cluster involved in degradation of substituted salicylates | Pseudomonas sp. |
5.5.1.1 | part of gene cluster involved in degradation of substituted salicylates | Pseudomonas reinekei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.1 | 0.0008 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.001 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.001 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0017 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0056 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0075 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0099 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0006 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0024 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0028 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0038 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0096 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0127 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0158 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 0.0158 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 0.011 | - |
3-methyl-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 0.026 | - |
3-methyl-cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 0.0306 | - |
2-methyl-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 0.031 | - |
cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 0.099 | - |
3-chloro-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 0.41 | - |
3-chloro-cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 1.73 | - |
cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.1 | 33000 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
1.13.11.1 | 34209 | - |
x * 34209, sequence calculation | Pseudomonas reinekei |
1.13.11.1 | 34233 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
1.14.13.1 | 48000 | - |
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
1.14.13.1 | 50712 | - |
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
5.5.1.1 | 40248 | - |
x * 40248, calculated | Pseudomonas reinekei |
5.5.1.1 | 41076 | - |
x * 41076, calculated, x * 42000, SDS-PAGE | Pseudomonas sp. |
5.5.1.1 | 42000 | - |
x * 41076, calculated, x * 42000, SDS-PAGE | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.1 | 4-methylcatechol + O2 | Pseudomonas reinekei | - |
(2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | Pseudomonas reinekei | - |
3-methyl-cis,cis-hexadienedioate | - |
? | |
1.14.13.1 | 4-methylsalicylate + NADH + 2 H+ + O2 | Pseudomonas reinekei | - |
4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-methylsalicylate + NADH + 2 H+ + O2 | Pseudomonas reinekei | - |
5-methylcatechol + NAD+ + H2O + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.1 | Pseudomonas reinekei | A0F0B5 | gene cat; encoded by the cat gene cluster, gene catA | - |
1.13.11.1 | Pseudomonas reinekei | Q0VH42 | gene salD; encoded by the cat gene cluster, gene catA | - |
1.14.13.1 | Pseudomonas reinekei | Q0VH44 | gene salA | - |
5.5.1.1 | Pseudomonas reinekei | Q4KPK2 | isoform SalC | - |
5.5.1.1 | Pseudomonas reinekei MT1 | Q4KPK2 | isoform SalC | - |
5.5.1.1 | Pseudomonas sp. | - |
isoform CatB | - |
5.5.1.1 | Pseudomonas sp. MT1 | - |
isoform CatB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.1 | native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration | Pseudomonas reinekei |
5.5.1.1 | purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells | Pseudomonas sp. |
5.5.1.1 | purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells | Pseudomonas reinekei |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.1 | culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
1.13.11.1 | culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | culture condition:4-methylsalicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | culture condition:5-methylsalicylate-grown cell | - |
Pseudomonas reinekei | - |
1.14.13.1 | culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.1 | 1.2 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
1.13.11.1 | 1.4 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
1.13.11.1 | 2.9 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
1.13.11.1 | 3.8 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
1.13.11.1 | 4.4 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
1.13.11.1 | 12.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
1.13.11.1 | 15.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
1.13.11.1 | 35.8 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
1.14.13.1 | 85 | - |
purified enzyme from 4-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
1.14.13.1 | 90 | - |
purified enzyme from 5-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
1.14.13.1 | 120 | - |
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate | Pseudomonas reinekei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.1 | 3-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | - |
Pseudomonas reinekei | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | preferred substrate | Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
1.13.11.1 | 4-methylcatechol + O2 | - |
Pseudomonas reinekei | 3-methyl-cis,cis-hexadienedioate | - |
? | |
1.13.11.1 | catechol + O2 | - |
Pseudomonas reinekei | cis,cis-muconate | - |
? | |
1.13.11.1 | additional information | no activity with 3-chlorocatechol | Pseudomonas reinekei | ? | - |
? | |
1.14.13.1 | 3-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 3-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 3-methylsalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 3-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 4-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 4-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 4-methylsalicylate + NADH + 2 H+ + O2 | - |
Pseudomonas reinekei | 4-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-chlorosalicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | 5-chlorocatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | 5-methylsalicylate + NADH + 2 H+ + O2 | - |
Pseudomonas reinekei | 5-methylcatechol + NAD+ + H2O + CO2 | - |
? | |
1.14.13.1 | salicylate + NADH + H+ + O2 | - |
Pseudomonas reinekei | catechol + NAD+ + H2O + CO2 | - |
? | |
5.5.1.1 | 2-chloro-cis,cis-muconate | - |
Pseudomonas sp. | 5-chloromuconolactone + 2-chloromuconolactone | - |
? | |
5.5.1.1 | 2-chloro-cis,cis-muconate | - |
Pseudomonas reinekei | 5-chloromuconolactone + 2-chloromuconolactone | - |
? | |
5.5.1.1 | 2-chloro-cis,cis-muconate | - |
Pseudomonas reinekei MT1 | 5-chloromuconolactone + 2-chloromuconolactone | - |
? | |
5.5.1.1 | 2-chloro-cis,cis-muconate | - |
Pseudomonas sp. MT1 | 5-chloromuconolactone + 2-chloromuconolactone | - |
? | |
5.5.1.1 | 2-methyl-cis,cis-muconate | - |
Pseudomonas sp. | 2-methylmuconolactone | - |
? | |
5.5.1.1 | 2-methyl-cis,cis-muconate | - |
Pseudomonas reinekei | 2-methylmuconolactone | - |
? | |
5.5.1.1 | 2-methyl-cis,cis-muconate | - |
Pseudomonas reinekei MT1 | 2-methylmuconolactone | - |
? | |
5.5.1.1 | 2-methyl-cis,cis-muconate | - |
Pseudomonas sp. MT1 | 2-methylmuconolactone | - |
? | |
5.5.1.1 | 3-Chloro-cis,cis-muconate | - |
Pseudomonas sp. | 5-Chloromuconolactone | - |
? | |
5.5.1.1 | 3-Chloro-cis,cis-muconate | - |
Pseudomonas reinekei | 5-Chloromuconolactone | - |
? | |
5.5.1.1 | 3-Chloro-cis,cis-muconate | - |
Pseudomonas reinekei MT1 | 5-Chloromuconolactone | - |
? | |
5.5.1.1 | 3-Chloro-cis,cis-muconate | - |
Pseudomonas sp. MT1 | 5-Chloromuconolactone | - |
? | |
5.5.1.1 | 3-methyl-cis,cis-muconate | - |
Pseudomonas sp. | 4-methylmuconolactone | - |
? | |
5.5.1.1 | 3-methyl-cis,cis-muconate | - |
Pseudomonas reinekei | 4-methylmuconolactone | - |
? | |
5.5.1.1 | 3-methyl-cis,cis-muconate | - |
Pseudomonas reinekei MT1 | 4-methylmuconolactone | - |
? | |
5.5.1.1 | 3-methyl-cis,cis-muconate | - |
Pseudomonas sp. MT1 | 4-methylmuconolactone | - |
? | |
5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas sp. | (+)-Muconolactone | - |
? | |
5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas reinekei | (+)-Muconolactone | - |
? | |
5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas reinekei MT1 | (+)-Muconolactone | - |
? | |
5.5.1.1 | cis,cis-Muconate | - |
Pseudomonas sp. MT1 | (+)-Muconolactone | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.1 | ? | x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
1.13.11.1 | ? | x * 34209, sequence calculation | Pseudomonas reinekei |
1.14.13.1 | ? | x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE | Pseudomonas reinekei |
5.5.1.1 | ? | x * 40248, calculated | Pseudomonas reinekei |
5.5.1.1 | ? | x * 41076, calculated, x * 42000, SDS-PAGE | Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.1 | C12O | - |
Pseudomonas reinekei |
1.14.13.1 | salicylate 1-hydroxylase | - |
Pseudomonas reinekei |
1.14.13.1 | salicylate hydroxylase | - |
Pseudomonas reinekei |
1.14.13.1 | SalOH | - |
Pseudomonas reinekei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.1 | 25 | - |
assay at | Pseudomonas reinekei |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.1 | 0.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.8 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 1.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 2.3 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 2.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 7.5 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 8.7 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 20.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 1.3 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 1.6 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 2.2 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 2.9 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 4.6 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 5.4 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 6.4 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 7.8 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 3 | 6 | 2-methyl-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 14.4 | - |
3-chloro-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 27.8 | - |
3-methyl-cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 120 | - |
3-chloro-cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 125 | - |
cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 186 | - |
cis,cis-muconate | isoform CatB, 25°C | Pseudomonas sp. | |
5.5.1.1 | 186 | - |
cis,cis-muconate | isoform CatB, 25°C | Pseudomonas reinekei | |
5.5.1.1 | 214 | - |
3-methyl-cis,cis-muconate | isoform SalC, 25°C | Pseudomonas reinekei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.1 | NADH | - |
Pseudomonas reinekei |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.13.1 | Pseudomonas reinekei | 5-chlorosalicylate induces the enzyme | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.1 | metabolism | 4- and 5-methylsalicylate as growth substrates are effectively converted by enzymes of the sal cluster into 4-methylmuconolactone, followed by isomerization to 3-methylmuconolactone. The function of the sal gene cluster is therefore to channel both chloro-substituted and methyl-substituted salicylates into a catechol ortho cleavage pathway, followed by dismantling of the formed substituted muconolactones through specific pathways | Pseudomonas reinekei |
1.13.11.1 | additional information | strain MT1 possesses two catechol 1,2-dioxygenases, encoded by the genes catA and salD, overview. The enzymes differ in their turnover rates for 4-chlorocatechol and especially for 4-methylcatechol, where C12OsalD exhibit significantly higher rates. Both enzymes also differ with respect to their apparent Km values | Pseudomonas reinekei |
1.14.13.1 | metabolism | salicylate 1-hydroxylase is not clustered with the meta cleavage pathway | Pseudomonas reinekei |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.1 | 0.0003 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0004 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0007 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0008 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.001 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0017 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0109 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.13.11.1 | 0.0122 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 170 | - |
3-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 340 | - |
NADH | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 340 | - |
3-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 410 | - |
4-methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 480 | - |
salicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 1000 | - |
4-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 2700 | - |
5-chlorosalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.14.13.1 | 3300 | - |
5-Methylsalicylate | pH not specified in the publication, 25°C | Pseudomonas reinekei |