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Literature summary extracted from

  • Camara, B.; Bielecki, P.; Kaminski, F.; dos Santos, V.M.; Plumeier, I.; Nikodem, P.; Pieper, D.H.
    A gene cluster involved in degradation of substituted salicylates via ortho cleavage in Pseudomonas sp. strain MT1 encodes enzymes specifically adapted for transformation of 4-methylcatechol and 3-methylmuconate (2007), J. Bacteriol., 189, 1664-1674.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 gene catA, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree, identification of the cat gene cluster of strain MT1 Pseudomonas reinekei
1.13.11.1 gene salD, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree Pseudomonas reinekei
1.14.13.1 gene salA, DNA and amino acid sequence determination and analysis, phylogenetic analysis Pseudomonas reinekei
5.5.1.1 part of gene cluster involved in degradation of substituted salicylates Pseudomonas sp.
5.5.1.1 part of gene cluster involved in degradation of substituted salicylates Pseudomonas reinekei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.0008
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.001
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.001
-
4-Chlorocatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0017
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0056
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0075
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0099
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0006
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0024
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0028
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0038
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0096
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0127
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0158
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 0.0158
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
5.5.1.1 0.011
-
3-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 0.026
-
3-methyl-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
5.5.1.1 0.0306
-
2-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 0.031
-
cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 0.099
-
3-chloro-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 0.41
-
3-chloro-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
5.5.1.1 1.73
-
cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 33000
-
x * 33000, SDS-PAGE, x * 34233, sequence calculation Pseudomonas reinekei
1.13.11.1 34209
-
x * 34209, sequence calculation Pseudomonas reinekei
1.13.11.1 34233
-
x * 33000, SDS-PAGE, x * 34233, sequence calculation Pseudomonas reinekei
1.14.13.1 48000
-
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei
1.14.13.1 50712
-
x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei
5.5.1.1 40248
-
x * 40248, calculated Pseudomonas reinekei
5.5.1.1 41076
-
x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.
5.5.1.1 42000
-
x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.1 4-methylcatechol + O2 Pseudomonas reinekei
-
(2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2 Pseudomonas reinekei
-
3-methyl-cis,cis-hexadienedioate
-
?
1.14.13.1 4-methylsalicylate + NADH + 2 H+ + O2 Pseudomonas reinekei
-
4-methylcatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 5-methylsalicylate + NADH + 2 H+ + O2 Pseudomonas reinekei
-
5-methylcatechol + NAD+ + H2O + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Pseudomonas reinekei A0F0B5 gene cat; encoded by the cat gene cluster, gene catA
-
1.13.11.1 Pseudomonas reinekei Q0VH42 gene salD; encoded by the cat gene cluster, gene catA
-
1.14.13.1 Pseudomonas reinekei Q0VH44 gene salA
-
5.5.1.1 Pseudomonas reinekei Q4KPK2 isoform SalC
-
5.5.1.1 Pseudomonas reinekei MT1 Q4KPK2 isoform SalC
-
5.5.1.1 Pseudomonas sp.
-
isoform CatB
-
5.5.1.1 Pseudomonas sp. MT1
-
isoform CatB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.1 native enzyme from 5-chlorosalicylate-grown cells by anion exchange chromatography and gel filtration Pseudomonas reinekei
5.5.1.1 purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells Pseudomonas sp.
5.5.1.1 purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells Pseudomonas reinekei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.1 culture condition:5-chlorosalicylate-grown cell
-
Pseudomonas reinekei
-
1.13.11.1 culture condition:salicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 culture condition:4-methylsalicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 culture condition:5-chlorosalicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 culture condition:5-methylsalicylate-grown cell
-
Pseudomonas reinekei
-
1.14.13.1 culture condition:salicylate-grown cell
-
Pseudomonas reinekei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 1.2
-
pH not specified in the publication, 25°C, substrate 4-chlorocatechol Pseudomonas reinekei
1.13.11.1 1.4
-
pH not specified in the publication, 25°C, substrate 3-methylcatechol Pseudomonas reinekei
1.13.11.1 2.9
-
pH not specified in the publication, 25°C, substrate 4-chlorocatechol Pseudomonas reinekei
1.13.11.1 3.8
-
pH not specified in the publication, 25°C, substrate 3-methylcatechol Pseudomonas reinekei
1.13.11.1 4.4
-
pH not specified in the publication, 25°C, substrate 4-methylcatechol Pseudomonas reinekei
1.13.11.1 12.3
-
pH not specified in the publication, 25°C, substrate catechol Pseudomonas reinekei
1.13.11.1 15.3
-
pH not specified in the publication, 25°C, substrate catechol Pseudomonas reinekei
1.13.11.1 35.8
-
pH not specified in the publication, 25°C, substrate 4-methylcatechol Pseudomonas reinekei
1.14.13.1 85
-
purified enzyme from 4-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
1.14.13.1 90
-
purified enzyme from 5-methylsalicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei
1.14.13.1 120
-
purified enzyme from salicylate-grown cells, pH not specified in the publication, 25°C, substrate salicylate Pseudomonas reinekei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
Pseudomonas reinekei (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-chlorocatechol + O2
-
Pseudomonas reinekei (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Pseudomonas reinekei (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2 preferred substrate Pseudomonas reinekei (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Pseudomonas reinekei 3-methyl-cis,cis-hexadienedioate
-
?
1.13.11.1 catechol + O2
-
Pseudomonas reinekei cis,cis-muconate
-
?
1.13.11.1 additional information no activity with 3-chlorocatechol Pseudomonas reinekei ?
-
?
1.14.13.1 3-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 3-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 3-methylsalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 3-methylcatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 4-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 4-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 4-methylsalicylate + NADH + 2 H+ + O2
-
Pseudomonas reinekei 4-methylcatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 5-chlorosalicylate + NADH + H+ + O2
-
Pseudomonas reinekei 5-chlorocatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 5-methylsalicylate + NADH + 2 H+ + O2
-
Pseudomonas reinekei 5-methylcatechol + NAD+ + H2O + CO2
-
?
1.14.13.1 salicylate + NADH + H+ + O2
-
Pseudomonas reinekei catechol + NAD+ + H2O + CO2
-
?
5.5.1.1 2-chloro-cis,cis-muconate
-
Pseudomonas sp. 5-chloromuconolactone + 2-chloromuconolactone
-
?
5.5.1.1 2-chloro-cis,cis-muconate
-
Pseudomonas reinekei 5-chloromuconolactone + 2-chloromuconolactone
-
?
5.5.1.1 2-chloro-cis,cis-muconate
-
Pseudomonas reinekei MT1 5-chloromuconolactone + 2-chloromuconolactone
-
?
5.5.1.1 2-chloro-cis,cis-muconate
-
Pseudomonas sp. MT1 5-chloromuconolactone + 2-chloromuconolactone
-
?
5.5.1.1 2-methyl-cis,cis-muconate
-
Pseudomonas sp. 2-methylmuconolactone
-
?
5.5.1.1 2-methyl-cis,cis-muconate
-
Pseudomonas reinekei 2-methylmuconolactone
-
?
5.5.1.1 2-methyl-cis,cis-muconate
-
Pseudomonas reinekei MT1 2-methylmuconolactone
-
?
5.5.1.1 2-methyl-cis,cis-muconate
-
Pseudomonas sp. MT1 2-methylmuconolactone
-
?
5.5.1.1 3-Chloro-cis,cis-muconate
-
Pseudomonas sp. 5-Chloromuconolactone
-
?
5.5.1.1 3-Chloro-cis,cis-muconate
-
Pseudomonas reinekei 5-Chloromuconolactone
-
?
5.5.1.1 3-Chloro-cis,cis-muconate
-
Pseudomonas reinekei MT1 5-Chloromuconolactone
-
?
5.5.1.1 3-Chloro-cis,cis-muconate
-
Pseudomonas sp. MT1 5-Chloromuconolactone
-
?
5.5.1.1 3-methyl-cis,cis-muconate
-
Pseudomonas sp. 4-methylmuconolactone
-
?
5.5.1.1 3-methyl-cis,cis-muconate
-
Pseudomonas reinekei 4-methylmuconolactone
-
?
5.5.1.1 3-methyl-cis,cis-muconate
-
Pseudomonas reinekei MT1 4-methylmuconolactone
-
?
5.5.1.1 3-methyl-cis,cis-muconate
-
Pseudomonas sp. MT1 4-methylmuconolactone
-
?
5.5.1.1 cis,cis-Muconate
-
Pseudomonas sp. (+)-Muconolactone
-
?
5.5.1.1 cis,cis-Muconate
-
Pseudomonas reinekei (+)-Muconolactone
-
?
5.5.1.1 cis,cis-Muconate
-
Pseudomonas reinekei MT1 (+)-Muconolactone
-
?
5.5.1.1 cis,cis-Muconate
-
Pseudomonas sp. MT1 (+)-Muconolactone
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 ? x * 33000, SDS-PAGE, x * 34233, sequence calculation Pseudomonas reinekei
1.13.11.1 ? x * 34209, sequence calculation Pseudomonas reinekei
1.14.13.1 ? x * 50712, sequence calculation, x * 48000, SalD, SDS-PAGE Pseudomonas reinekei
5.5.1.1 ? x * 40248, calculated Pseudomonas reinekei
5.5.1.1 ? x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C12O
-
Pseudomonas reinekei
1.14.13.1 salicylate 1-hydroxylase
-
Pseudomonas reinekei
1.14.13.1 salicylate hydroxylase
-
Pseudomonas reinekei
1.14.13.1 SalOH
-
Pseudomonas reinekei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.1 25
-
assay at Pseudomonas reinekei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.1 0.7
-
4-Chlorocatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.8
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 1.7
-
4-Chlorocatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 2.3
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 2.7
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 7.5
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 8.7
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 20.7
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 1.3
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 1.6
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 2.2
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 2.9
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 4.6
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 5.4
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 6.4
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 7.8
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
5.5.1.1 3 6 2-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 14.4
-
3-chloro-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 27.8
-
3-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 120
-
3-chloro-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
5.5.1.1 125
-
cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
5.5.1.1 186
-
cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
5.5.1.1 186
-
cis,cis-muconate isoform CatB, 25°C Pseudomonas reinekei
5.5.1.1 214
-
3-methyl-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.1 NADH
-
Pseudomonas reinekei

Expression

EC Number Organism Comment Expression
1.14.13.1 Pseudomonas reinekei 5-chlorosalicylate induces the enzyme up

General Information

EC Number General Information Comment Organism
1.13.11.1 metabolism 4- and 5-methylsalicylate as growth substrates are effectively converted by enzymes of the sal cluster into 4-methylmuconolactone, followed by isomerization to 3-methylmuconolactone. The function of the sal gene cluster is therefore to channel both chloro-substituted and methyl-substituted salicylates into a catechol ortho cleavage pathway, followed by dismantling of the formed substituted muconolactones through specific pathways Pseudomonas reinekei
1.13.11.1 additional information strain MT1 possesses two catechol 1,2-dioxygenases, encoded by the genes catA and salD, overview. The enzymes differ in their turnover rates for 4-chlorocatechol and especially for 4-methylcatechol, where C12OsalD exhibit significantly higher rates. Both enzymes also differ with respect to their apparent Km values Pseudomonas reinekei
1.14.13.1 metabolism salicylate 1-hydroxylase is not clustered with the meta cleavage pathway Pseudomonas reinekei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.1 0.0003
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0004
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0007
-
4-Chlorocatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0008
-
3-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.001
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0017
-
4-Chlorocatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0109
-
catechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.13.11.1 0.0122
-
4-methylcatechol pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 170
-
3-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 340
-
NADH pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 340
-
3-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 410
-
4-methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 480
-
salicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 1000
-
4-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 2700
-
5-chlorosalicylate pH not specified in the publication, 25°C Pseudomonas reinekei
1.14.13.1 3300
-
5-Methylsalicylate pH not specified in the publication, 25°C Pseudomonas reinekei