EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.104 | molecular biology | analyses of conditions to metabolize exogenously supplied cyanate | Synechocystis sp. |
4.2.1.104 | molecular biology | analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the Cyn-ABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), low internal pools of HCO3- and CO2 result in an insufficient supply of bicarbonate | Synechococcus elongatus |
4.2.1.104 | molecular biology | analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), inactivation of the cynS gene leads to inability of decomposition of external cyanate | Synechococcus elongatus |
4.2.1.104 | molecular biology | analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the CynABDS operon, light, and on activity of the CO2-concentrating mechanism (CCM), mutagensis of a periplasmatic binding protein of a multicomponent ABC-transporter (CynA), leads to inability of decomposition of external cyanate due to impaired cyanate uptake, cyanase function is not affected | Synechococcus elongatus |
4.2.1.104 | molecular biology | analyses of conditions to metabolize exogenously supplied cyanate, depending on proteins of the operon CynABDS, light and internal pools of HCO3- and CO2 | Synechococcus sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.104 | expressed in Escherichia coli | Synechococcus elongatus |
4.2.1.104 | expressed in Escherichia coli | Synechococcus sp. |
4.2.1.104 | expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster | Synechocystis sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.104 | additional information | targeted mutants of Synechococcus elongatus strain PCC7942 disrupted in the coding sequences of the gene encoding the cytosoliyc cyanase (CynS) to study metabolism of exogenously supplied cyanate | Synechococcus elongatus |
4.2.1.104 | additional information | targeted mutants of Synechococcus elongatus strain PCC7942 impaired in the CO2-concentrating mechanism CCM to study metabolism of exogenously supplied cyanate in cyanobacteria, inactivation of CCM impairs metabolism of external cyanate by insufficient internal pools of HCO3- and CO2 resulting in low levels of the co-substrate bicarbonate that is required for cyanase activity | Synechococcus elongatus |
4.2.1.104 | additional information | targeted mutants of Synechococcus elongatus strain PCC7942, disruption of the cynA gene, encoding a binding protein of a multicomponent ABC-transporter, inactivation of CynA does not affect cyanase function but inhibits decomposition of external cyanate | Synechococcus elongatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.104 | oxalate | the membrane-impermeant inhibitor of cyanobacterial cyanase has minor effects on acid-labile or acid-stable fractions indicating the absence of cyanase from the periplasm | Synechococcus elongatus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.104 | cytosol | - |
Synechococcus sp. | 5829 | - |
4.2.1.104 | cytosol | - |
Synechococcus elongatus | 5829 | - |
4.2.1.104 | cytosol | the cytosolic cyanase is encoded by the cynS gene, part of the operon CynABDS | Synechococcus elongatus | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.104 | cyanate + HCO3- + 2 H+ | Synechococcus sp. | - |
NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | Synechococcus elongatus | - |
NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | Synechococcus elongatus PCC 7942 | - |
NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | Synechococcus sp. UTEX625 | - |
NH3 + 2 CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechococcus elongatus | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechococcus sp. | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechocystis sp. | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechococcus elongatus | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH4+ + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechococcus elongatus PCC 7942 | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | Synechococcus elongatus PCC 7942 | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | NH4+ + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.104 | Synechococcus elongatus | - |
strain PCC7942 | - |
4.2.1.104 | Synechococcus elongatus | Q59948 | strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon | - |
4.2.1.104 | Synechococcus elongatus | Q59948 | strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase | - |
4.2.1.104 | Synechococcus elongatus | Q59948 | strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM) | - |
4.2.1.104 | Synechococcus elongatus PCC 7942 | - |
strain PCC7942 | - |
4.2.1.104 | Synechococcus elongatus PCC 7942 | Q59948 | strain PCC7942, CynA mutant, the cynA gene encodes a binding protein of an ABC transporter, part of the CynABDS operon | - |
4.2.1.104 | Synechococcus elongatus PCC 7942 | Q59948 | strain PCC7942, CynS mutant, disruption of the cynS gene encoding cyanase | - |
4.2.1.104 | Synechococcus elongatus PCC 7942 | Q59948 | strain PCC7942, mutant DELTA-chpX-DELTA-chpY, disturbed activity of the CO2-concentrating mechanism (CCM) | - |
4.2.1.104 | Synechococcus sp. | - |
- |
- |
4.2.1.104 | Synechococcus sp. | Q5N0J1 | strain UTEX625, also known as Synechococcus sp. strain PCC6301 | - |
4.2.1.104 | Synechococcus sp. UTEX625 | - |
- |
- |
4.2.1.104 | Synechocystis sp. | Q55367 | strain PCC6803 | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.104 | additional information | - |
cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light | Synechocystis sp. |
4.2.1.104 | additional information | - |
cyanase activity shown in whole-cell extracts of the photoautotrophic Synechococcus sp. strain UTEX 625, ability to utilize cyanate-derived CO2 to support photosynthesis affected | Synechococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.104 | cyanate + HCO3- + 2 H+ | - |
Synechococcus sp. | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | - |
Synechococcus elongatus | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | - |
Synechococcus elongatus PCC 7942 | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | cyanate + HCO3- + 2 H+ | - |
Synechococcus sp. UTEX625 | NH3 + 2 CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechococcus elongatus | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechococcus sp. | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechocystis sp. | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate in cyanobacteria | Synechocystis sp. | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate in photoautotroph cyanobacteria | Synechococcus sp. | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 | Synechococcus elongatus | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechococcus elongatus PCC 7942 | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 | Synechococcus elongatus PCC 7942 | NH3 + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechococcus elongatus | NH4+ + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | utilization of exogenous cyanate as a niche source of C and N in cyanobacteria | Synechococcus elongatus PCC 7942 | NH4+ + CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 | Synechococcus elongatus | NH4+ + 2 CO2 | - |
? | |
4.2.1.104 | NCO- + HCO3- + H+ | studies on decomposition of externally supplied cyanate, depending on the CynABDS operon, light and internal pools of HCO3- and CO2 | Synechococcus elongatus PCC 7942 | NH4+ + 2 CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.104 | cyanase | - |
Synechococcus elongatus |
4.2.1.104 | cyanase | - |
Synechococcus sp. |
4.2.1.104 | cyanase | - |
Synechocystis sp. |