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Literature summary extracted from

  • Heinhorst, S.; Williams, E.B.; Cai, F.; Murin, C.D.; Shively, J.M.; Cannon, G.C.
    Characterization of the carboxysomal carbonic anhydrase CsoSCA from Halothiobacillus neapolitanus (2006), J. Bacteriol., 188, 8087-8094.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expression in Escherichia coli Halothiobacillus neapolitanus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 2-mercaptoethanol
-
 Halothiobacillus neapolitanus
4.2.1.1 azide
-
 Halothiobacillus neapolitanus
4.2.1.1 dithiothreitol
-
 Halothiobacillus neapolitanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 3.2
-
 CO2 rCsoSCA Halothiobacillus neapolitanus
4.2.1.1 9.3
-
 HCO3- rCsoSCA Halothiobacillus neapolitanus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 carboxysome
-
 Halothiobacillus neapolitanus 31470
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ addition of 1 mM ZnCl2 to the metal-depleted enzyme restores the ctivity to nearly the level observed for nondialyzed samples, while neither CoCl2 nor MgCl2 is able to reverse the inactivation of the enzyme. Zn2+ contents of 0.9 mol of Zn2+ per mol of monomer Halothiobacillus neapolitanus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Halothiobacillus neapolitanus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant Halothiobacillus neapolitanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Halothiobacillus neapolitanus H2CO3
-
 r
4.2.1.1 additional information rCsoSCA exhibits no detectable esterase activity when assayed spectrophotometrically with 4-nitrophenylacetate as a substrate Halothiobacillus neapolitanus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 CsoSCA
-
 Halothiobacillus neapolitanus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 46000
-
 HCO3- pH 7.0, rCsoSCA Halothiobacillus neapolitanus
4.2.1.1 89000
-
 CO2 rCsoSCA Halothiobacillus neapolitanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 6 7.5 kcat for dehydration increases over the pH range of 6.0 to 7.5 Halothiobacillus neapolitanus
4.2.1.1 6.5 8.5 CO2 hydration increases over a pH range from 6.5 to 8.5 Halothiobacillus neapolitanus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.26
-
-
 Halothiobacillus neapolitanus dithiothreitol
4.2.1.1 3.4
-
-
 Halothiobacillus neapolitanus 2-mercaptoethanol
4.2.1.1 4.1
-
-
 Halothiobacillus neapolitanus azide