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Literature summary extracted from

  • Bose, A.; Pritchett, M.A.; Rother, M.; Metcalf, W.W.
    Differential regulation of the three methanol methyltransferase isozymes in Methanosarcina acetivorans C2A (2006), J. Bacteriol., 188, 7274-7283.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.249 additional information analysis of the metal content of MtbB1 by plasma emission spectroscopy revealed no detectable cobalt with a lower limit of detection for cobalt of 0.04 mol of cobalt/mol of polypeptide Methanosarcina barkeri
2.1.1.249 Zn2+ 0.43 mol of zinc/mol of polypeptide Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.249 51000
-
 x * 51000, SDS-PAGE Methanosarcina barkeri
2.1.1.249 230000
-
 gel filtration Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.90 additional information Methanosarcina acetivorans analysis of mtaCB gene regulation in Methanosarcina acetivorans ?
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 additional information Methanosarcina barkeri MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine. A MtbB1to corrinoid protein MtbC ratio of 1 is optimal for coenzyme M methylation with dimethylamine. MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimethylamine, indicating MtbB1 carries an active site for dimethylamine demethylation and corrinoid methylation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina acetivorans
-
-
-
2.1.1.249 Methanosarcina barkeri
-
 gene mtbB1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.249 native enzyme 35.2fold MtbB1 by two steps of anion exchange chromatography, and adsorption and hydrophobic interaction chromatography Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.249 1.89
-
 purified enzyme, pH 6.5, 23°C Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 additional information analysis of mtaCB gene regulation in Methanosarcina acetivorans Methanosarcina acetivorans ?
-
 ?
2.1.1.249 dimethylamine + a [Co(I) dimethylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine
-
 ?
2.1.1.249 additional information MtbB1 demethylates dimethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequently demethylated by MtbA to methylate coenzyme M during methanogenesis from dimethylamine. A MtbB1to corrinoid protein MtbC ratio of 1 is optimal for coenzyme M methylation with dimethylamine. MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimethylamine, indicating MtbB1 carries an active site for dimethylamine demethylation and corrinoid methylation Methanosarcina barkeri ?
-
 ?
2.1.1.249 additional information dimethylamine-dependent methylation of free cobalamin by MtbB1, kinetics, overview Methanosarcina barkeri ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.249 oligomer x * 51000, SDS-PAGE Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
2.1.1.90 methanol:5-hydroxy-benzimidazolylcobamide methyltransferase
-
 Methanosarcina acetivorans
2.1.1.90 MtaB
-
 Methanosarcina acetivorans
2.1.1.249 dimethylamine: corrinoid methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 DMA:corrinoid methyltransferase
-
 Methanosarcina barkeri
2.1.1.249 MtbB1
-
 Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.249 23
-
 assay at Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.249 6.5
-
 assay at Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.249 metabolism highly purified dimethylamine: corrinoid methyltransferase MtbB1, corrinoid protein MtbC, and methylcorrinoid:coenzyme M methyltransferase MtbA, EC 2.1.1.247, are sufficient to catalyze in vitro CoM methylation by DMA Methanosarcina barkeri
2.1.1.249 additional information MtbB1 is a 230-kDa protein composed of 51-kDa subunits that do not possess a corrinoid prosthetic group Methanosarcina barkeri