EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.266 | expressed in Escherichia coli | Methanococcoides burtonii |
3.1.3.85 | expressed in Escherichia coli | Methanococcoides burtonii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.266 | 0.54 | - |
3-phospho-D-glycerate | pH 7.6, 30°C | Methanococcoides burtonii | |
2.4.1.266 | 1.6 | - |
3-phospho-D-glycerate | pH 7.6, 50°C | Methanococcoides burtonii | |
2.4.1.266 | 1.75 | - |
GDP-glucose | pH 7.6, 30°C | Methanococcoides burtonii | |
2.4.1.266 | 3.7 | - |
GDP-glucose | pH 7.6, 50°C | Methanococcoides burtonii | |
3.1.3.85 | 0.08 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 30°C | Methanococcoides burtonii | |
3.1.3.85 | 0.1 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 30°C | Thermus thermophilus | |
3.1.3.85 | 0.11 | - |
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | pH 7.6, 30°C | Thermus thermophilus | |
3.1.3.85 | 0.27 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 50°C | Methanococcoides burtonii | |
3.1.3.85 | 0.28 | - |
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | pH 7.6, 50°C | Methanococcoides burtonii | |
3.1.3.85 | 0.36 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 50°C | Thermus thermophilus | |
3.1.3.85 | 0.37 | - |
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | pH 7.6, 50°C | Thermus thermophilus | |
3.1.3.85 | 0.79 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 70°C | Thermus thermophilus | |
3.1.3.85 | 0.83 | - |
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | pH 7.6, 70°C | Thermus thermophilus | |
3.1.3.85 | 0.9 | - |
2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | pH 7.6, 90°C | Thermus thermophilus | |
3.1.3.85 | 1.1 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.6, 90°C | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.266 | Co2+ | strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM | Methanococcoides burtonii | |
2.4.1.266 | Mg2+ | strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+ | Methanococcoides burtonii | |
2.4.1.266 | Mn2+ | strictly dependent on divalent cations in the following order of efficiency: Mn2+, Co2+, Mg2+. Maximal activity at 1 mM | Methanococcoides burtonii | |
2.4.1.266 | additional information | K+, Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate GpgS activity at any concentration | Methanococcoides burtonii | |
3.1.3.85 | Co2+ | at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. However, when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate, 3% activity is detected without cations, and the preferred metal ion with both substrates is Co2+ (10 mM). At 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+ | Methanococcoides burtonii | |
3.1.3.85 | K+ | at 30°C, GpgP retains 37% of its enzyme activity towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate and 12% activity towards 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate in a reaction mixture without cations compared to the activity with 10 mM K+ and Co2+. Potassium chloride, in the concentration range of 10 to 100 mM, stimulates enzyme activity about 40% at 50°C, but only when 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate is the substrate. However, at 30°C, K+ has a stimulatory effect towards 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate, but not with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate as the substrate | Methanococcoides burtonii | |
3.1.3.85 | Mg2+ | at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ | Methanococcoides burtonii | |
3.1.3.85 | Mn2+ | enzyme activity is not detected with Mn2+ when 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is used, while Mn2+ had a strong stimulatory effect (85% of the maximal activity with Co2+) with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate | Methanococcoides burtonii | |
3.1.3.85 | additional information | Ca2+, Sr2+, Ba2+, Cu2+, and Zn2+, have no effect on GpgP activity at any concentration | Methanococcoides burtonii | |
3.1.3.85 | Ni2+ | at 50°C, divalent cations are absolutely required for GpgP activity with 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate as the substrate. Mg2+ and, to a lesser extent, Ni2+ can replace Co2+ | Methanococcoides burtonii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 43000 | - |
x * 43000, calculated from sequence | Methanococcoides burtonii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | Methanococcoides burtonii | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | Methanococcoides burtonii | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | Thermus thermophilus | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.266 | Methanococcoides burtonii | Q12XX4 | - |
- |
3.1.3.85 | Methanococcoides burtonii | - |
- |
- |
3.1.3.85 | Thermus thermophilus | Q84B23 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.266 | recombinant enzyme | Methanococcoides burtonii |
3.1.3.85 | recombinant enzyme | Methanococcoides burtonii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 0.0116 | - |
pH 7.6, 30°C | Methanococcoides burtonii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | - |
Methanococcoides burtonii | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP). An mpgS gene coding for mannosyl-3-phosphoglycerate synthase (MpgS) is absent | Methanococcoides burtonii | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | additional information | no activity with: ADP-ribose, UDP-acetylgalactosamine, UDP-glucuronic acid, UDP-galactose, GDP-fucose, GDP-mannose, UDP-mannose, ADP-mannose, UDP-glucose, TDP-glucose, and ADP-glucose as sugar donors and glycerol, D-2-phosphoglycerate, L-glycerol-3-phosphate, 2,3-diphospho-D-glycerate, and phosphoenolpyruvate | Methanococcoides burtonii | ? | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) | Methanococcoides burtonii | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | synthesis of the solute glucosylglycerate proceeds via a two-step pathway involving glucosyl-3-phosphoglycerate synthase (GpgS) and glucosyl-3-phosphoglycerate phosphatase (GpgP) | Thermus thermophilus | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C | Thermus thermophilus | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate | Methanococcoides burtonii | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similar to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C | Thermus thermophilus | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate | - |
? | |
3.1.3.85 | 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.266 | ? | x * 43000, calculated from sequence | Methanococcoides burtonii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.266 | GpgS | - |
Methanococcoides burtonii |
3.1.3.85 | GpgP | - |
Methanococcoides burtonii |
3.1.3.85 | GpgP | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 50 | - |
- |
Methanococcoides burtonii |
3.1.3.85 | 50 | - |
substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
3.1.3.85 | 60 | - |
substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 30 | 70 | 30°C: about 50% of maximal activity, 70°C: about 50% of maximal activity | Methanococcoides burtonii |
3.1.3.85 | 30 | 70 | 30°C: about 55% of maximal activity, 70°C: about 50% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
3.1.3.85 | 50 | 70 | 50°C: about 60% of maximal activity, 70°C: about 60% of maximal activity, substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 4 | - |
half-life: 51.3 h | Methanococcoides burtonii |
2.4.1.266 | 23 | - |
half-life: 11.5 h | Methanococcoides burtonii |
2.4.1.266 | 50 | - |
half-life: 13.8 h | Methanococcoides burtonii |
3.1.3.85 | 4 | - |
half-life: 198 h | Methanococcoides burtonii |
3.1.3.85 | 23 | - |
half-life: 165 h | Methanococcoides burtonii |
3.1.3.85 | 50 | - |
half-life: 12 min | Methanococcoides burtonii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 7.5 | 8.5 | - |
Methanococcoides burtonii |
3.1.3.85 | 5.5 | - |
substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
3.1.3.85 | 6.5 | - |
substrate: 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 6.5 | 9.5 | pH 6.5: about 55% of maximal activity, pH 9.5: about 70% of maximal activity | Methanococcoides burtonii |
3.1.3.85 | 4.5 | 9 | pH 4.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity, substrate: 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.85 | additional information | - |
additional information | the Vmax/Km-value for 2-O-(alpha-D-glucopyranisyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 30°C and at 50°C. The Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is similat to the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 70°C and at 90°C | Thermus thermophilus | |
3.1.3.85 | additional information | - |
additional information | the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is about 2fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Methanococcoides burtonii |