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Literature summary extracted from

  • Lower, B.H.; Potters, M.B.; Kennelly, P.J.
    A phosphoprotein from the archaeon Sulfolobus solfataricus with protein-serine/threonine kinase activity (2004), J. Bacteriol., 186, 463-472.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 ORF sso0469, amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.1 D349A site-directed mutagenesis, inactive mutant Saccharolobus solfataricus
2.7.11.1 D394A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-tpe enzyme Saccharolobus solfataricus
2.7.11.1 K215A site-directed mutagenesis, inactive mutant Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 tamoxifen
-
 Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 membrane associated Saccharolobus solfataricus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ less effective than Mn2+ Saccharolobus solfataricus
2.7.11.1 Mn2+ preferred metal ion cofactor Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 67000
-
 x * 67000, glycosylated and phosphorylated PK3, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + a protein Saccharolobus solfataricus
-
 ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + a protein Saccharolobus solfataricus P2
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Saccharolobus solfataricus
-
 ORF sso0469 encodes PK3
-
2.7.11.1 Saccharolobus solfataricus P2
-
 ORF sso0469 encodes PK3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 native enzyme partially by detergent solublization from membranes and anion exchange chromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + a protein
-
 Saccharolobus solfataricus ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + a protein phosphorylation of exogenous substrates takes place on serine and, occasionally, threonine, but the enzyme displays a strong preference for threonine as the phospho-acceptor amino acid residue, overview, no activity with GTP except for the autophosphorylation reaction in vitro which functions with GTP and also GDP Saccharolobus solfataricus ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + a protein
-
 Saccharolobus solfataricus P2 ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + a protein phosphorylation of exogenous substrates takes place on serine and, occasionally, threonine, but the enzyme displays a strong preference for threonine as the phospho-acceptor amino acid residue, overview, no activity with GTP except for the autophosphorylation reaction in vitro which functions with GTP and also GDP Saccharolobus solfataricus P2 ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + bovine serum albumin
-
 Saccharolobus solfataricus ADP + phosphorylated bovine serum albumin
-
 ?
2.7.11.1 ATP + bovine serum albumin
-
 Saccharolobus solfataricus P2 ADP + phosphorylated bovine serum albumin
-
 ?
2.7.11.1 ATP + casein
-
 Saccharolobus solfataricus ADP + phosphocasein
-
 ?
2.7.11.1 ATP + casein
-
 Saccharolobus solfataricus P2 ADP + phosphocasein
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Saccharolobus solfataricus ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.1 ATP + myelin basic protein
-
 Saccharolobus solfataricus P2 ADP + phosphorylated myelin basic protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 ? x * 67000, glycosylated and phosphorylated PK3, SDS-PAGE Saccharolobus solfataricus
2.7.11.1 More enzyme domain structure analysis Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 membrane-associated protein-serine/threonine kinase
-
 Saccharolobus solfataricus
2.7.11.1 SsoPK3
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 35
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 6.5
-
 assay at Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Saccharolobus solfataricus
2.7.11.1 additional information no activity with GTP Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.1 Saccharolobus solfataricus PK3, isoelectric focusing
-
 5.7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.11.1 0.5
-
 pH 6.5, 35°C, recombinant enzyme Saccharolobus solfataricus tamoxifen