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Literature summary extracted from

  • Lower, B.H.; Kennelly, P.J.
    Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity (2003), J. Bacteriol., 185, 3436-3445.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.1 orf sso2387, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 EDTA
-
 Saccharolobus solfataricus
2.7.11.1 tamoxifen
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Ca2+ can only poorly substitute for Mn2+ Saccharolobus solfataricus
2.7.11.1 Mg2+ less effective than Mn2+ Saccharolobus solfataricus
2.7.11.1 Mn2+ preferred metal ion cofactor, especially for the autophosphorylation reaction Saccharolobus solfataricus
2.7.11.1 Ni2+ can only poorly substitute for Mn2+ Saccharolobus solfataricus
2.7.11.1 Zn2+ can only partially substitute for Mn2+ Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.1 ATP + a protein Saccharolobus solfataricus
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Saccharolobus solfataricus
-
 orf sso2387
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein the enzyme performs autophosphorylation at Ser548, determination of sites of autophosphorylation by mass spectrometry in two areas, one immediately N terminal to the region corresponding to subdomain I of eukaryotic protein kinases, and the second N terminal to the presumed activation loop located between subdomains VII and VIII, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C Saccharolobus solfataricus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.11.1 solubilization from inclusion bodies after recombinant expression in Escherichia coli by treatment with 5 M urea Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 additional information
-
 substrate specificity of the recombinant enzyme, overview Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ATP + a protein
-
 Saccharolobus solfataricus ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + a protein PK2 phosphorylate itself as well as several exogenous proteins, including mixed histones, casein, bovine serum albumin, and reduced carboxyamidomethylated and maleylated lysozyme, on serine residues, overview, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C Saccharolobus solfataricus ADP + a phosphoprotein
-
 ?
2.7.11.1 ATP + bovine serum albumin
-
 Saccharolobus solfataricus ADP + phosphorylated bovine serum albumin
-
 ?
2.7.11.1 ATP + casein
-
 Saccharolobus solfataricus ADP + phosphocasein
-
 ?
2.7.11.1 ATP + histone
-
 Saccharolobus solfataricus ADP + phosphorylated histone
-
 ?
2.7.11.1 ATP + lysozyme reduced carboxyamidomethylated and maleylated lysozyme Saccharolobus solfataricus ADP + phopshorylated lysozyme
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.1 SsoPK2
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 37
-
 phosophorylation of exogenous proteins Saccharolobus solfataricus
2.7.11.1 60
-
 above, autophosphorylation activity Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.1 ATP
-
 Saccharolobus solfataricus